Title |
A new sensitive PCR assay for one-step detection of 12 IDH1/2 mutations in glioma
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Published in |
Acta Neuropathologica Communications, June 2014
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DOI | 10.1186/2051-5960-2-58 |
Pubmed ID | |
Authors |
Aurélie Catteau, Hélène Girardi, Florence Monville, Cécile Poggionovo, Sabrina Carpentier, Véronique Frayssinet, Jesse Voss, Robert Jenkins, Blandine Boisselier, Karima Mokhtari, Marc Sanson, Hélène Peyro-Saint-Paul, Caterina Giannini |
Abstract |
Mutations in isocitrate dehydrogenase genes IDH1 or IDH2 are frequent in glioma, and IDH mutation status is a strong diagnostic and prognostic marker. Current IDH mutation screening is performed with an immunohistochemistry (IHC) assay specific for IDH1 R132H, the most common mutation. Sequencing is recommended as a second-step test for IHC-negative or -equivocal cases. We developed and validated a new real-time quantitative polymerase chain reaction (PCR) assay for single-step detection of IDH1 R132H and 11 rare IDH1/2 mutations in formalin-fixed paraffin-embedded (FFPE) glioma samples. Performance of the IDH1/2 PCR assay was compared to IHC and Sanger sequencing. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 2% |
Unknown | 43 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 16% |
Researcher | 7 | 16% |
Student > Doctoral Student | 5 | 11% |
Student > Bachelor | 5 | 11% |
Other | 3 | 7% |
Other | 9 | 20% |
Unknown | 8 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 17 | 39% |
Medicine and Dentistry | 12 | 27% |
Agricultural and Biological Sciences | 3 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 2% |
Nursing and Health Professions | 1 | 2% |
Other | 0 | 0% |
Unknown | 10 | 23% |