Title |
Optimized DNA extraction from neonatal dried blood spots: application in methylome profiling
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Published in |
BMC Biotechnology, July 2014
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DOI | 10.1186/1472-6750-14-60 |
Pubmed ID | |
Authors |
Akram Ghantous, Richard Saffery, Marie-Pierre Cros, Anne-Louise Ponsonby, Steven Hirschfeld, Carol Kasten, Terence Dwyer, Zdenko Herceg, Hector Hernandez-Vargas |
Abstract |
Neonatal dried blood spots (DBS) represent an inexpensive method for long-term biobanking worldwide and are considered gold mines for research for several human diseases, including those of metabolic, infectious, genetic and epigenetic origin. However, the utility of DBS is restricted by the limited amount and quality of extractable biomolecules (including DNA), especially for genome wide profiling. Degradation of DNA in DBS often occurs during storage and extraction. Moreover, amplifying small quantities of DNA often leads to a bias in subsequent data, particularly in methylome profiles. Thus it is important to develop methodologies that maximize both the yield and quality of DNA from DBS for downstream analyses. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 83% |
Scientists | 1 | 17% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 1% |
Netherlands | 1 | 1% |
Norway | 1 | 1% |
Unknown | 84 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 17 | 20% |
Student > Master | 14 | 16% |
Researcher | 12 | 14% |
Student > Bachelor | 7 | 8% |
Professor | 6 | 7% |
Other | 15 | 17% |
Unknown | 16 | 18% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 22 | 25% |
Biochemistry, Genetics and Molecular Biology | 15 | 17% |
Medicine and Dentistry | 8 | 9% |
Chemistry | 5 | 6% |
Engineering | 4 | 5% |
Other | 15 | 17% |
Unknown | 18 | 21% |