Title |
Improved sugar co-utilisation by encapsulation of a recombinant Saccharomyces cerevisiae strain in alginate-chitosan capsules
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Published in |
Biotechnology for Biofuels and Bioproducts, July 2014
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DOI | 10.1186/1754-6834-7-102 |
Pubmed ID | |
Authors |
Johan O Westman, Nicklas Bonander, Mohammad J Taherzadeh, Carl Johan Franzén |
Abstract |
Two major hurdles for successful production of second-generation bioethanol are the presence of inhibitory compounds in lignocellulosic media, and the fact that Saccharomyces cerevisiae cannot naturally utilise pentoses. There are recombinant yeast strains that address both of these issues, but co-utilisation of glucose and xylose is still an issue that needs to be resolved. A non-recombinant way to increase yeast tolerance to hydrolysates is by encapsulation of the yeast. This can be explained by concentration gradients occuring in the cell pellet inside the capsule. In the current study, we hypothesised that encapsulation might also lead to improved simultaneous utilisation of hexoses and pentoses because of such sugar concentration gradients. |
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