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Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation

Overview of attention for article published in BMC Veterinary Research, August 2017
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4 tweeters

Citations

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54 Mendeley
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Title
Detection and measurement of alpha-amylase in canine saliva and changes after an experimentally induced sympathetic activation
Published in
BMC Veterinary Research, August 2017
DOI 10.1186/s12917-017-1191-4
Pubmed ID
Authors

María Dolores Contreras-Aguilar, Fernando Tecles, Silvia Martínez-Subiela, Damián Escribano, Luis Jesús Bernal, José Joaquín Cerón

Abstract

Salivary alpha-amylase (sAA) is considered a biomarker of sympathetic activation in humans, but there is controversy regarding the existence of sAA in dogs. The hypothesis of this study was that sAA exists in dogs and it could change in situations of sympathetic stimulation. Therefore, the aims of this study were: 1) to demonstrate the presence of alpha-amylase in saliva of dogs by Western-Blot, 2) to validate an spectrophotometric method for the measurement of sAA activity and 3) to evaluate the possible changes in sAA activity after the induction of an ejaculation in dogs which is known to produce a sympathetic activation. Western-Blot demonstrated a band in dog saliva specimens between 60 kDa and 50 kDa, similar to purified sAA. The spectrophotometric assay validated showed an adequate inter- and intra-assay precision, and a high correlation coefficient (r = 0.999) in the linearity under dilution study. sAA median activity significantly increased just after ejaculation compared with just before the ejaculation (2.06-fold, P = 0.005). This study demonstrated the existence of alpha-amylase in saliva of dogs and that this enzyme can be measured by a spectrophotometric assay. In addition, results showed that sAA increase after a sympathetic activation and could be potentially used as non-invasive biomarker of sympathetic activity in this species.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 54 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 19%
Student > Bachelor 10 19%
Student > Master 7 13%
Student > Doctoral Student 5 9%
Student > Postgraduate 3 6%
Other 8 15%
Unknown 11 20%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 19 35%
Agricultural and Biological Sciences 9 17%
Engineering 2 4%
Biochemistry, Genetics and Molecular Biology 2 4%
Chemistry 2 4%
Other 6 11%
Unknown 14 26%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2022.
All research outputs
#13,391,391
of 22,721,584 outputs
Outputs from BMC Veterinary Research
#935
of 3,037 outputs
Outputs of similar age
#159,411
of 316,505 outputs
Outputs of similar age from BMC Veterinary Research
#28
of 76 outputs
Altmetric has tracked 22,721,584 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,037 research outputs from this source. They receive a mean Attention Score of 3.8. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,505 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.