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microRNAs participate in gene expression regulation and phytohormone cross-talk in barley embryo during seed development and germination

Overview of attention for article published in BMC Plant Biology, September 2017
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Title
microRNAs participate in gene expression regulation and phytohormone cross-talk in barley embryo during seed development and germination
Published in
BMC Plant Biology, September 2017
DOI 10.1186/s12870-017-1095-2
Pubmed ID
Authors

Bin Bai, Bo Shi, Ning Hou, Yanli Cao, Yijun Meng, Hongwu Bian, Muyuan Zhu, Ning Han

Abstract

Small RNA and degradome sequencing have identified a large number of miRNA-target pairs in plant seeds. However, detailed spatial and temporal studies of miRNA-mediated regulation, which can reflect links between seed development and germination are still lacking. In this study, we extended our investigation on miRNAs-involved gene regulation by a combined analysis of seed maturation and germination in barley. Through bioinformatics analysis of small RNA sequencing data, a total of 1324 known miRNA families and 448 novel miRNA candidates were identified. Of those, 16 known miRNAs with 40 target genes, and three novel miRNAs with four target genes were confirmed based on degradome sequencing data. Conserved miRNA families such as miR156, miR168, miR166, miR167, and miR894 were highly expressed in embryos of developing and germinating seeds. A barley-specific miRNA, miR5071, which was predicted to target an OsMLA10-like gene, accumulated at a high level, suggesting its involvement in defence response during these two developmental stages. Based on target prediction and Kyoto Encyclopedia of Genes and Genomes analysis of putative targets, nine highly expressed miRNAs were found to be related to phytohormone signalling and hormone cross-talk. Northern blot and qRT-PCR analysis showed that these miRNAs displayed differential expression patterns during seed development and germination, indicating their different roles in hormone signalling pathways. In addition, we showed that miR393 affected seed development through targeting two genes encoding the auxin receptors TIR1/AFBs in barley, as over-expression of miR393 led to an increased length-width ratio of seeds, whereas target mimic (MIM393)-mediated inhibition of its activity decreased the 1000-grain weight of seeds. Furthermore, the expression of auxin-responsive genes, abscisic acid- and gibberellic acid-related genes was altered in miR393 misexpression lines during germination and early seedling growth. Our work indicates that miRNA-target pairs participate in gene expression regulation and hormone interaction in barley embryo and provides evidence that miR393-mediated auxin response regulation affects grain development and influences gibberellic acid and abscisic acid homeostasis during germination.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 59 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 32%
Researcher 11 19%
Student > Master 6 10%
Student > Bachelor 5 8%
Unspecified 3 5%
Other 4 7%
Unknown 11 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 23 39%
Biochemistry, Genetics and Molecular Biology 18 31%
Unspecified 3 5%
Environmental Science 1 2%
Engineering 1 2%
Other 0 0%
Unknown 13 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2017.
All research outputs
#20,446,373
of 23,001,641 outputs
Outputs from BMC Plant Biology
#2,547
of 3,282 outputs
Outputs of similar age
#275,643
of 315,600 outputs
Outputs of similar age from BMC Plant Biology
#21
of 26 outputs
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