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A simple and cost-effective method of DNA extraction from small formalin-fixed paraffin-embedded tissue for molecular oncologic testing

Overview of attention for article published in BMC Clinical Pathology, July 2014
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  • Among the highest-scoring outputs from this source (#44 of 116)
  • Average Attention Score compared to outputs of the same age

Mentioned by

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3 X users

Citations

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25 Dimensions

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88 Mendeley
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Title
A simple and cost-effective method of DNA extraction from small formalin-fixed paraffin-embedded tissue for molecular oncologic testing
Published in
BMC Clinical Pathology, July 2014
DOI 10.1186/1472-6890-14-30
Pubmed ID
Authors

Anthony N Snow, Aaron A Stence, Jonathan A Pruessner, Aaron D Bossler, Deqin Ma

Abstract

Extraction of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is a critical step in molecular oncologic testing. As molecular oncology testing becomes more important for prognostic and therapeutic decision making and tissue specimens become smaller due to earlier detection of suspicious lesions and the use of fine needle aspiration methods for tissue collection, it becomes more challenging for the typical molecular pathology laboratory to obtain reliable test results. We developed a DNA extraction method to obtain sufficient quantity and high quality genomic DNA from limited FFPE tissue for molecular oncology testing using a combination of H&E stained slides, a matrix capture method and the Qiagen DNA column.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 1%
Canada 1 1%
Unknown 86 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 19 22%
Student > Ph. D. Student 14 16%
Student > Bachelor 13 15%
Student > Master 12 14%
Other 6 7%
Other 12 14%
Unknown 12 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 26 30%
Biochemistry, Genetics and Molecular Biology 18 20%
Medicine and Dentistry 14 16%
Veterinary Science and Veterinary Medicine 3 3%
Nursing and Health Professions 2 2%
Other 12 14%
Unknown 13 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 October 2014.
All research outputs
#13,410,148
of 22,758,248 outputs
Outputs from BMC Clinical Pathology
#44
of 116 outputs
Outputs of similar age
#108,778
of 225,737 outputs
Outputs of similar age from BMC Clinical Pathology
#6
of 8 outputs
Altmetric has tracked 22,758,248 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 116 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 62% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 225,737 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.