Title |
A simple and cost-effective method of DNA extraction from small formalin-fixed paraffin-embedded tissue for molecular oncologic testing
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Published in |
BMC Clinical Pathology, July 2014
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DOI | 10.1186/1472-6890-14-30 |
Pubmed ID | |
Authors |
Anthony N Snow, Aaron A Stence, Jonathan A Pruessner, Aaron D Bossler, Deqin Ma |
Abstract |
Extraction of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue is a critical step in molecular oncologic testing. As molecular oncology testing becomes more important for prognostic and therapeutic decision making and tissue specimens become smaller due to earlier detection of suspicious lesions and the use of fine needle aspiration methods for tissue collection, it becomes more challenging for the typical molecular pathology laboratory to obtain reliable test results. We developed a DNA extraction method to obtain sufficient quantity and high quality genomic DNA from limited FFPE tissue for molecular oncology testing using a combination of H&E stained slides, a matrix capture method and the Qiagen DNA column. |
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