Title |
Whole-genome sequencing of clarithromycin resistant Helicobacter pylori characterizes unidentified variants of multidrug resistant efflux pump genes
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Published in |
Gut Pathogens, June 2014
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DOI | 10.1186/1757-4749-6-27 |
Pubmed ID | |
Authors |
Akira Iwamoto, Toshihito Tanahashi, Rina Okada, Yukio Yoshida, Kaoru Kikuchi, Yoshihide Keida, Yoshiki Murakami, Lin Yang, Koji Yamamoto, Shin Nishiumi, Masaru Yoshida, Takeshi Azuma |
Abstract |
Clarithromycin (CLR) is the key drug in eradication therapy of Helicobacter pylori (H. pylori) infection, and widespread use of CLR has led to an increase in primary CLR-resistant H. pylori. The known mechanism of CLR resistance has been established in A2146G and A2147G mutations in the 23S rRNA gene, but evidence of the involvement of other genetic mechanisms is lacking. Using the MiSeq platform, whole-genome sequencing of the 19 clinical strains and the reference strain ATCC26695 was performed to identify single nucleotide variants (SNVs) of multi-drug resistant efflux pump genes in the CLR-resistant phenotype. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Venezuela, Bolivarian Republic of | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 41 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 17% |
Student > Master | 6 | 15% |
Student > Postgraduate | 4 | 10% |
Researcher | 3 | 7% |
Other | 2 | 5% |
Other | 7 | 17% |
Unknown | 12 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 17% |
Medicine and Dentistry | 7 | 17% |
Agricultural and Biological Sciences | 6 | 15% |
Immunology and Microbiology | 6 | 15% |
Nursing and Health Professions | 1 | 2% |
Other | 1 | 2% |
Unknown | 13 | 32% |