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Mendeley readers
| Title |
Selection of reference genes for quantitative RT-PCR studies in striped dolphin (Stenella coeruleoalba) skin biopsies
|
|---|---|
| Published in |
BMC Molecular and Cell Biology, September 2006
|
| DOI | 10.1186/1471-2199-7-32 |
| Pubmed ID | |
| Authors |
Giacomo Spinsanti, Cristina Panti, Elisa Lazzeri, Letizia Marsili, Silvia Casini, Francesco Frati, Cristina Maria Fossi |
| Abstract |
Odontocete cetaceans occupy the top position of the marine food-web and are particularly sensitive to the bioaccumulation of lipophilic contaminants. The effects of environmental pollution on these species are highly debated and various ecotoxicological studies have addressed the impact of xenobiotic compounds on marine mammals, raising conservational concerns. Despite its sensitivity, quantitative real-time PCR (qRT-PCR) has never been used to quantify gene induction caused by exposure of cetaceans to contaminants. A limitation for the application of qRT-PCR is the need for appropriate reference genes which allow the correct quantification of gene expression. A systematic evaluation of potential reference genes in cetacean skin biopsies is presented, in order to validate future qRT-PCR studies aiming at using the expression of selected genes as non-lethal biomarkers. |
Mendeley readers
The data shown below were compiled from readership statistics for 114 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 3% |
| Italy | 1 | <1% |
| Canada | 1 | <1% |
| Sweden | 1 | <1% |
| Spain | 1 | <1% |
| Slovenia | 1 | <1% |
| Unknown | 106 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 23 | 20% |
| Researcher | 23 | 20% |
| Student > Master | 16 | 14% |
| Student > Bachelor | 9 | 8% |
| Student > Postgraduate | 8 | 7% |
| Other | 23 | 20% |
| Unknown | 12 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 68 | 60% |
| Biochemistry, Genetics and Molecular Biology | 9 | 8% |
| Environmental Science | 8 | 7% |
| Veterinary Science and Veterinary Medicine | 6 | 5% |
| Medicine and Dentistry | 2 | 2% |
| Other | 7 | 6% |
| Unknown | 14 | 12% |