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Acquisition of resistance to avian leukosis virus subgroup B through mutations on tvb cysteine-rich domains in DF-1 chicken fibroblasts

Overview of attention for article published in Veterinary Research, September 2017
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2 tweeters

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23 Mendeley
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Title
Acquisition of resistance to avian leukosis virus subgroup B through mutations on tvb cysteine-rich domains in DF-1 chicken fibroblasts
Published in
Veterinary Research, September 2017
DOI 10.1186/s13567-017-0454-1
Pubmed ID
Authors

Hong Jo Lee, Kyung Youn Lee, Young Hyun Park, Hee Jung Choi, Yongxiu Yao, Venugopal Nair, Jae Yong Han

Abstract

Avian leukosis virus (ALV) is a retrovirus that causes tumors in avian species, and its vertical and horizontal transmission in poultry flocks results in enormous economic losses. Despite the discovery of specific host receptors, there have been few reports on the modulation of viral susceptibility via genetic modification. We therefore engineered acquired resistance to ALV subgroup B using CRISPR/Cas9-mediated genome editing technology in DF-1 chicken fibroblasts. Using this method, we efficiently modified the tumor virus locus B (tvb) gene, encoding the TVB receptor, which is essential for ALV subgroup B entry into host cells. By expanding individual DF-1 clones, we established that artificially generated premature stop codons in the cysteine-rich domain (CRD) of TVB receptor confer resistance to ALV subgroup B. Furthermore, we found that a cysteine residue (C80) of CRD2 plays a crucial role in ALV subgroup B entry. These results suggest that CRISPR/Cas9-mediated genome editing can be used to efficiently modify avian cells and establish novel chicken cell lines with resistance to viral infection.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 17%
Student > Ph. D. Student 3 13%
Researcher 3 13%
Student > Doctoral Student 2 9%
Other 2 9%
Other 5 22%
Unknown 4 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 30%
Agricultural and Biological Sciences 6 26%
Veterinary Science and Veterinary Medicine 2 9%
Unspecified 1 4%
Immunology and Microbiology 1 4%
Other 2 9%
Unknown 4 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2018.
All research outputs
#9,917,451
of 12,387,983 outputs
Outputs from Veterinary Research
#598
of 794 outputs
Outputs of similar age
#197,286
of 268,654 outputs
Outputs of similar age from Veterinary Research
#6
of 9 outputs
Altmetric has tracked 12,387,983 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 794 research outputs from this source. They receive a mean Attention Score of 3.5. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 268,654 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 3 of them.