Title |
Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci
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Published in |
Epigenetics & Chromatin, April 2014
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DOI | 10.1186/1756-8935-7-8 |
Pubmed ID | |
Authors |
Daniel Smeets, Yolanda Markaki, Volker J Schmid, Felix Kraus, Anna Tattermusch, Andrea Cerase, Michael Sterr, Susanne Fiedler, Justin Demmerle, Jens Popken, Heinrich Leonhardt, Neil Brockdorff, Thomas Cremer, Lothar Schermelleh, Marion Cremer |
Abstract |
A Xist RNA decorated Barr body is the structural hallmark of the compacted inactive X territory in female mammals. Using super-resolution three-dimensional structured illumination microscopy (3D-SIM) and quantitative image analysis, we compared its ultrastructure with active chromosome territories (CTs) in human and mouse somatic cells, and explored the spatio-temporal process of Barr body formation at onset of inactivation in early differentiating mouse embryonic stem cells (ESCs). |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Hungary | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 3 | 1% |
United Kingdom | 3 | 1% |
Germany | 2 | <1% |
France | 2 | <1% |
Canada | 1 | <1% |
Poland | 1 | <1% |
Unknown | 224 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 61 | 26% |
Researcher | 43 | 18% |
Student > Bachelor | 35 | 15% |
Student > Master | 21 | 9% |
Professor | 11 | 5% |
Other | 31 | 13% |
Unknown | 34 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 87 | 37% |
Biochemistry, Genetics and Molecular Biology | 72 | 31% |
Physics and Astronomy | 11 | 5% |
Engineering | 7 | 3% |
Computer Science | 5 | 2% |
Other | 18 | 8% |
Unknown | 36 | 15% |