Title |
Gene delivery in mouse auditory brainstem and hindbrain using in utero electroporation
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Published in |
Molecular Brain, July 2014
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DOI | 10.1186/s13041-014-0051-4 |
Pubmed ID | |
Authors |
Laurence S David, Jamila Aitoubah, Lee Stephen Lesperance, Lu-Yang Wang |
Abstract |
Manipulation of gene expression via recombinant viral vectors and creation of transgenic knock-out/in animals has revolutionized our understanding of genes that play critical roles during neuronal development and pathophysiology of neurological disorders. Recently, target-specific genetic manipulations are made possible to perform in combination with specific Cre-lines, albeit costly, labor-intensive and time consuming. Thus, alternative methods of gene manipulations to address important biological questions are highly desirable. In this study, we utilized in utero electroporation technique which involves efficient delivery of hindbrain-specific enhancer/promoter construct, Krox20 into the third ventricle of live mouse embryo to investigate green fluorescent protein (GFP) expression pattern in mouse auditory brainstem and other hindbrain neurons. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 50% |
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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France | 2 | 5% |
United Kingdom | 1 | 3% |
Germany | 1 | 3% |
Unknown | 33 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 7 | 19% |
Researcher | 7 | 19% |
Student > Master | 6 | 16% |
Professor > Associate Professor | 3 | 8% |
Student > Bachelor | 2 | 5% |
Other | 6 | 16% |
Unknown | 6 | 16% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 12 | 32% |
Neuroscience | 8 | 22% |
Medicine and Dentistry | 4 | 11% |
Nursing and Health Professions | 3 | 8% |
Biochemistry, Genetics and Molecular Biology | 1 | 3% |
Other | 1 | 3% |
Unknown | 8 | 22% |