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Gene delivery in mouse auditory brainstem and hindbrain using in utero electroporation

Overview of attention for article published in Molecular Brain, July 2014
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Mentioned by

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2 tweeters

Citations

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5 Dimensions

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37 Mendeley
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Title
Gene delivery in mouse auditory brainstem and hindbrain using in utero electroporation
Published in
Molecular Brain, July 2014
DOI 10.1186/s13041-014-0051-4
Pubmed ID
Authors

Laurence S David, Jamila Aitoubah, Lee Stephen Lesperance, Lu-Yang Wang

Abstract

Manipulation of gene expression via recombinant viral vectors and creation of transgenic knock-out/in animals has revolutionized our understanding of genes that play critical roles during neuronal development and pathophysiology of neurological disorders. Recently, target-specific genetic manipulations are made possible to perform in combination with specific Cre-lines, albeit costly, labor-intensive and time consuming. Thus, alternative methods of gene manipulations to address important biological questions are highly desirable. In this study, we utilized in utero electroporation technique which involves efficient delivery of hindbrain-specific enhancer/promoter construct, Krox20 into the third ventricle of live mouse embryo to investigate green fluorescent protein (GFP) expression pattern in mouse auditory brainstem and other hindbrain neurons.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 2 5%
United Kingdom 1 3%
Germany 1 3%
Unknown 33 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 19%
Researcher 7 19%
Student > Master 6 16%
Professor > Associate Professor 3 8%
Student > Bachelor 2 5%
Other 6 16%
Unknown 6 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 32%
Neuroscience 8 22%
Medicine and Dentistry 4 11%
Nursing and Health Professions 3 8%
Biochemistry, Genetics and Molecular Biology 1 3%
Other 1 3%
Unknown 8 22%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 July 2014.
All research outputs
#4,385,474
of 8,250,328 outputs
Outputs from Molecular Brain
#263
of 444 outputs
Outputs of similar age
#86,850
of 180,552 outputs
Outputs of similar age from Molecular Brain
#15
of 20 outputs
Altmetric has tracked 8,250,328 research outputs across all sources so far. This one is in the 27th percentile – i.e., 27% of other outputs scored the same or lower than it.
So far Altmetric has tracked 444 research outputs from this source. They receive a mean Attention Score of 4.2. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 180,552 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 20 others from the same source and published within six weeks on either side of this one. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.