Title |
Circumventing qPCR inhibition to amplify miRNAs in plasma
|
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Published in |
Biomarker Research, July 2014
|
DOI | 10.1186/2050-7771-2-13 |
Pubmed ID | |
Abstract |
Circulating microRNAs (c-miRNAs) have be identified in saliva, urine and blood, which has led to increasing interest in their development as biomarkers for diverse diseases including cancers. One of the key advantages of c-miRNAs over other biomarkers is the ability to be amplified and quantified by quantitative PCR (qPCR). However, at phlebotomy when whole blood is dispensed into heparinized tubes, residual levels of the anti-coagulant lithium heparin may remain in the plasma and hence with RNA isolated from the plasma. This can confound the detection of c-miRNAs by qPCR because it inhibits reverse transcriptase (RT). Here we present a procedure, modified from earlier techniques, to detect c-miRNAs in plasma that improves sensitivity and streamlines performance. |
X Demographics
Geographical breakdown
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
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Denmark | 1 | 2% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 11 | 20% |
Student > Ph. D. Student | 8 | 15% |
Student > Master | 8 | 15% |
Other | 5 | 9% |
Student > Bachelor | 4 | 7% |
Other | 6 | 11% |
Unknown | 12 | 22% |
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Biochemistry, Genetics and Molecular Biology | 11 | 20% |
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Immunology and Microbiology | 2 | 4% |
Engineering | 2 | 4% |
Other | 2 | 4% |
Unknown | 13 | 24% |