Title |
PAT-ChIP coupled with laser microdissection allows the study of chromatin in selected cell populations from paraffin-embedded patient samples
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Published in |
Epigenetics & Chromatin, August 2014
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DOI | 10.1186/1756-8935-7-18 |
Pubmed ID | |
Authors |
Stefano Amatori, Marco Ballarini, Alice Faversani, Elena Belloni, Fulvia Fusar, Silvano Bosari, Pier Giuseppe Pelicci, Saverio Minucci, Mirco Fanelli |
Abstract |
The recent introduction of pathology tissue-chromatin immunoprecipitation (PAT-ChIP), a technique allowing chromatin immunoprecipitation from formalin-fixed and paraffin-embedded (FFPE) tissues, has expanded the application potential of epigenetic studies in tissue samples. However, FFPE tissue section analysis is strongly limited by tissue heterogeneity, which hinders linking the observed epigenetic events to the corresponding cellular population. Thus, ideally, to take full advantage of PAT-ChIP approaches, procedures able to increase the purity and homogeneity of cell populations from FFPE tissues are required. |
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