Title |
Development of duplex PCR for differential detection of goatpox and sheeppox viruses
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Published in |
BMC Veterinary Research, August 2017
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DOI | 10.1186/s12917-017-1179-0 |
Pubmed ID | |
Authors |
Zhixun Zhao, Guohua Wu, Xinmin Yan, Xueliang Zhu, Jian Li, Haixia Zhu, Zhidong Zhang, Qiang Zhang |
Abstract |
Clinically, sheeppox and goatpox have the same symptoms and cannot be distinguished serologically. A cheaper and easy method for differential diagnosis is important in control of this disease in endemic region. A duplex PCR assay was developed for the specific differential detection of Goatpox virus (GTPV) and Sheeppox virus (SPPV), using two sets of primers based on viral E10R gene and RPO132 gene. Nucleic acid electrophoresis results showed that SPPV-positive samples appear two bands, and GTPV-positive samples only one stripe. There were no cross-reactions with nucleic acids extracted from other pathogens including foot-and-mouth disease virus, Orf virus. The duplex PCR assay developed can specially detect SPPV or GTPV present in samples (n = 135) collected from suspected cases of Capripox. The duplex PCR assay developed is a specific and sensitive method for the differential diagnosis of GTPV and SPPV infection, with the potential to be standardized as a detection method for Capripox in endemic areas. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Canada | 1 | 20% |
Unknown | 4 | 80% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 80% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 4 | 20% |
Unspecified | 2 | 10% |
Student > Bachelor | 2 | 10% |
Student > Ph. D. Student | 2 | 10% |
Professor | 1 | 5% |
Other | 2 | 10% |
Unknown | 7 | 35% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 4 | 20% |
Immunology and Microbiology | 3 | 15% |
Unspecified | 2 | 10% |
Agricultural and Biological Sciences | 2 | 10% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Other | 3 | 15% |
Unknown | 5 | 25% |