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Development of duplex PCR for differential detection of goatpox and sheeppox viruses

Overview of attention for article published in BMC Veterinary Research, August 2017
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  • Above-average Attention Score compared to outputs of the same age and source (60th percentile)

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Title
Development of duplex PCR for differential detection of goatpox and sheeppox viruses
Published in
BMC Veterinary Research, August 2017
DOI 10.1186/s12917-017-1179-0
Pubmed ID
Authors

Zhixun Zhao, Guohua Wu, Xinmin Yan, Xueliang Zhu, Jian Li, Haixia Zhu, Zhidong Zhang, Qiang Zhang

Abstract

Clinically, sheeppox and goatpox have the same symptoms and cannot be distinguished serologically. A cheaper and easy method for differential diagnosis is important in control of this disease in endemic region. A duplex PCR assay was developed for the specific differential detection of Goatpox virus (GTPV) and Sheeppox virus (SPPV), using two sets of primers based on viral E10R gene and RPO132 gene. Nucleic acid electrophoresis results showed that SPPV-positive samples appear two bands, and GTPV-positive samples only one stripe. There were no cross-reactions with nucleic acids extracted from other pathogens including foot-and-mouth disease virus, Orf virus. The duplex PCR assay developed can specially detect SPPV or GTPV present in samples (n = 135) collected from suspected cases of Capripox. The duplex PCR assay developed is a specific and sensitive method for the differential diagnosis of GTPV and SPPV infection, with the potential to be standardized as a detection method for Capripox in endemic areas.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 20%
Unspecified 2 10%
Student > Bachelor 2 10%
Student > Ph. D. Student 2 10%
Professor 1 5%
Other 2 10%
Unknown 7 35%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 4 20%
Immunology and Microbiology 3 15%
Unspecified 2 10%
Agricultural and Biological Sciences 2 10%
Biochemistry, Genetics and Molecular Biology 1 5%
Other 3 15%
Unknown 5 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 March 2018.
All research outputs
#13,570,270
of 23,002,898 outputs
Outputs from BMC Veterinary Research
#943
of 3,065 outputs
Outputs of similar age
#159,934
of 316,368 outputs
Outputs of similar age from BMC Veterinary Research
#27
of 73 outputs
Altmetric has tracked 23,002,898 research outputs across all sources so far. This one is in the 39th percentile – i.e., 39% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,065 research outputs from this source. They receive a mean Attention Score of 3.9. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,368 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 73 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.