Title |
An integrated analysis of the SOX2 microRNA response program in human pluripotent and nullipotent stem cell lines
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Published in |
BMC Genomics, August 2014
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DOI | 10.1186/1471-2164-15-711 |
Pubmed ID | |
Authors |
Sebastian F Vencken, Praveen Sethupathy, Gordon Blackshields, Cathy Spillane, Salah Elbaruni, Orla Sheils, Michael F Gallagher, John J O’Leary |
Abstract |
SOX2 is a core component of the transcriptional network responsible for maintaining embryonal carcinoma cells (ECCs) in a pluripotent, undifferentiated state of self-renewal. As such, SOX2 is an oncogenic transcription factor and crucial cancer stem cell (CSC) biomarker in embryonal carcinoma and, as more recently found, in the stem-like cancer cell component of many other malignancies. SOX2 is furthermore a crucial factor in the maintenance of adult stem cell phenotypes and has additional roles in cell fate determination. The SOX2-linked microRNA (miRNA) transcriptome and regulome has not yet been fully defined in human pluripotent cells or CSCs. To improve our understanding of the SOX2-linked miRNA regulatory network as a contribution to the phenotype of these cell types, we used high-throughput differential miRNA and gene expression analysis combined with existing genome-wide SOX2 chromatin immunoprecipitation (ChIP) data to map the SOX2 miRNA transcriptome in two human embryonal carcinoma cell (hECC) lines. |
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Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
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France | 1 | 2% |
Unknown | 44 | 96% |
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Student > Ph. D. Student | 9 | 20% |
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Researcher | 7 | 15% |
Student > Postgraduate | 3 | 7% |
Other | 5 | 11% |
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Psychology | 1 | 2% |
Other | 0 | 0% |
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