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The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli

Overview of attention for article published in BMC Microbiology, September 2017
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Title
The role of the two-component systems Cpx and Arc in protein alterations upon gentamicin treatment in Escherichia coli
Published in
BMC Microbiology, September 2017
DOI 10.1186/s12866-017-1100-9
Pubmed ID
Authors

Emina Ćudić, Kristin Surmann, Gianna Panasia, Elke Hammer, Sabine Hunke

Abstract

The aminoglycoside antibiotic gentamicin was supposed to induce a crosstalk between the Cpx- and the Arc-two-component systems (TCS). Here, we investigated the physical interaction of the respective TCS components and compared the results with their respective gene expression and protein abundance. The findings were interpreted in relation to the global proteome profile upon gentamicin treatment. We observed specific interaction between CpxA and ArcA upon treatment with the aminoglycoside gentamicin using Membrane-Strep-tagged protein interaction experiments (mSPINE). This interaction was neither accompanied by detectable phosphorylation of ArcA nor by activation of the Arc system via CpxA. Furthermore, no changes in absolute amounts of the Cpx- and Arc-TCS could be determined with the sensitive single reaction monitoring (SRM) in presence of gentamicin. Nevertheless, upon applying shotgun mass spectrometry analysis after treatment with gentamicin, we observed a reduction of ArcA ~ P-dependent protein synthesis and a significant Cpx-dependent alteration in the global proteome profile of E. coli. This study points to the importance of the Cpx-TCS within the complex regulatory network in the E. coli response to aminoglycoside-caused stress.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 8 24%
Student > Master 5 15%
Researcher 4 12%
Student > Ph. D. Student 4 12%
Other 3 9%
Other 2 6%
Unknown 8 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 32%
Agricultural and Biological Sciences 6 18%
Immunology and Microbiology 4 12%
Chemistry 2 6%
Computer Science 1 3%
Other 2 6%
Unknown 8 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2018.
All research outputs
#9,748,558
of 12,719,839 outputs
Outputs from BMC Microbiology
#1,175
of 1,873 outputs
Outputs of similar age
#181,002
of 268,892 outputs
Outputs of similar age from BMC Microbiology
#1
of 1 outputs
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