@TanushJagdish I prepare electrocomp cells and electroporate Pseudomonas and Francisella at room temp and it always works. Reference paper for Pseudomonas: https://t.co/PEcHVTO3KJ
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@pseudo_papers any advice on this protocol PA community? https://t.co/HiT33Du6kA I found odd that the reference for growing PAO1 at 42ºC O/N without shaking doesn't exist/match any previous protocols. They suggest using this temperature because it inhibits
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RT @EvolvedBiofilm: A rapid seamless method for gene knockout in Pseudomonas aeruginosa https://t.co/DIiORX40Wr
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RT @EvolvedBiofilm: A rapid seamless method for gene knockout in Pseudomonas aeruginosa https://t.co/DIiORX40Wr