Title |
Rifampicin resistance mutations in the 81 bp RRDR of rpoB gene in Mycobacterium tuberculosis clinical isolates using Xpert®MTB/RIF in Kampala, Uganda: a retrospective study
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Published in |
BMC Infectious Diseases, September 2014
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DOI | 10.1186/1471-2334-14-481 |
Pubmed ID | |
Authors |
Gerald Mboowa, Carolyn Namaganda, Willy Ssengooba |
Abstract |
Introduction of Xpert® MTB/RIF assay has revolutionalised the diagnosis of tuberculosis (TB) by simultaneously detecting the bacteria and resistance to rifampicin (rif), a surrogate marker for multi-drug resistant TB (MDR-TB) as well as one of the principal first-line anti-tuberculosis drugs. In general, rpoB mutations can be found in 96.1% of rif-resistant Mycobacterium tuberculosis (MTB) strains worldwide and these mutations usually are located in a region at the 507-533rd amino acid residuals (81 bp) in the MTB rpoB gene, which is referred to as Rifampicin-resistance-determining region (RRDR). In this study, we determined the frequency of MDR-TB in Kampala using Xpert® MTB/RIF in comparison with the agar proportion method using Middlebrook 7H11and further determined the frequency of probes for different rpoB gene mutations using Xpert® MTB/RIF assay in the 81 bp RRDR. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Comoros | 1 | 17% |
United States | 1 | 17% |
Uganda | 1 | 17% |
Unknown | 3 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 6 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | <1% |
Unknown | 221 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 70 | 32% |
Student > Master | 18 | 8% |
Student > Ph. D. Student | 16 | 7% |
Researcher | 14 | 6% |
Other | 12 | 5% |
Other | 17 | 8% |
Unknown | 75 | 34% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 97 | 44% |
Agricultural and Biological Sciences | 15 | 7% |
Biochemistry, Genetics and Molecular Biology | 9 | 4% |
Immunology and Microbiology | 9 | 4% |
Nursing and Health Professions | 4 | 2% |
Other | 10 | 5% |
Unknown | 78 | 35% |