Title |
Gene expression studies for the analysis of domoic acid production in the marine diatom Pseudo-nitzschia multiseries
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Published in |
BMC Molecular and Cell Biology, November 2013
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DOI | 10.1186/1471-2199-14-25 |
Pubmed ID | |
Authors |
Katie Rose Boissonneault, Brooks M Henningsen, Stephen S Bates, Deborah L Robertson, Sean Milton, Jerry Pelletier, Deborah A Hogan, David E Housman |
Abstract |
Pseudo-nitzschia multiseries Hasle (Hasle) (Ps-n) is distinctive among the ecologically important marine diatoms because it produces the neurotoxin domoic acid. Although the biology of Ps-n has been investigated intensely, the characterization of the genes and biochemical pathways leading to domoic acid biosynthesis has been limited. To identify transcripts whose levels correlate with domoic acid production, we analyzed Ps-n under conditions of high and low domoic acid production by cDNA microarray technology and reverse-transcription quantitative PCR (RT-qPCR) methods. Our goals included identifying and validating robust reference genes for Ps-n RNA expression analysis under these conditions. |
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Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Mexico | 1 | 2% |
United States | 1 | 2% |
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Student > Doctoral Student | 4 | 6% |
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Other | 0 | 0% |
Unknown | 15 | 23% |