Title |
Genome-wide expression assay comparison across frozen and fixed postmortem brain tissue samples
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Published in |
BMC Genomics, September 2011
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DOI | 10.1186/1471-2164-12-449 |
Pubmed ID | |
Authors |
Maggie L Chow, Hai-Ri Li, Mary E Winn, Craig April, Cynthia C Barnes, Anthony Wynshaw-Boris, Jian-Bing Fan, Xiang-Dong Fu, Eric Courchesne, Nicholas J Schork |
Abstract |
Gene expression assays have been shown to yield high quality genome-wide data from partially degraded RNA samples. However, these methods have not yet been applied to postmortem human brain tissue, despite their potential to overcome poor RNA quality and other technical limitations inherent in many assays. We compared cDNA-mediated annealing, selection, and ligation (DASL)- and in vitro transcription (IVT)-based genome-wide expression profiling assays on RNA samples from artificially degraded reference pools, frozen brain tissue, and formalin-fixed brain tissue. |
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