Title |
Dissecting a neuron network: FIB-SEM-based 3D-reconstruction of the visual neuropils in the sea spider Achelia langi (Dohrn, 1881) (Pycnogonida)
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Published in |
BMC Biology, August 2014
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DOI | 10.1186/s12915-014-0059-3 |
Pubmed ID | |
Authors |
Tobias Lehmann, Martin Heß, Gerhard Wanner, Roland R Melzer |
Abstract |
The research field of connectomics arose just recently with the development of new three-dimensional-electron microscopy (EM) techniques and increasing computing power. So far, only a few model species (for example, mouse, the nematode Caenorhabditis elegans, and the fruit fly Drosophila melanogaster) have been studied using this approach. Here, we present a first attempt to expand this circle to include pycnogonids, which hold a key position for the understanding of arthropod evolution. The visual neuropils in Achelia langi are studied using a focused ion beam-scanning electron microscope (FIB-SEM) crossbeam-workstation, and a three-dimensional serial reconstruction of the connectome is presented. |
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