Title |
Single molecule sequencing and genome assembly of a clinical specimen of Loa loa,the causative agent of loiasis
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Published in |
BMC Genomics, September 2014
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DOI | 10.1186/1471-2164-15-788 |
Pubmed ID | |
Authors |
Luke J Tallon, Xinyue Liu, Sasisekhar Bennuru, Marcus C Chibucos, Alvaro Godinez, Sandra Ott, Xuechu Zhao, Lisa Sadzewicz, Claire M Fraser, Thomas B Nutman, Julie C Dunning Hotopp |
Abstract |
More than 20% of the world's population is at risk for infection by filarial nematodes and >180 million people worldwide are already infected. Along with infection comes significant morbidity that has a socioeconomic impact. The eight filarial nematodes that infect humans are Wuchereria bancrofti, Brugia malayi, Brugia timori, Onchocerca volvulus, Loa loa, Mansonella perstans, Mansonella streptocerca, and Mansonella ozzardi, of which three have published draft genome sequences. Since all have humans as the definitive host, standard avenues of research that rely on culturing and genetics have often not been possible. Therefore, genome sequencing provides an important window into understanding the biology of these parasites. The need for large amounts of high quality genomic DNA from homozygous, inbred lines; the availability of only short sequence reads from next-generation sequencing platforms at a reasonable expense; and the lack of random large insert libraries has limited our ability to generate high quality genome sequences for these parasites. However, the Pacific Biosciences single molecule, real-time sequencing platform holds great promise in reducing input amounts and generating sufficiently long sequences that bypass the need for large insert paired libraries. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 7 | 25% |
United Kingdom | 6 | 21% |
Canada | 2 | 7% |
Mexico | 1 | 4% |
China | 1 | 4% |
Belgium | 1 | 4% |
India | 1 | 4% |
France | 1 | 4% |
Unknown | 8 | 29% |
Demographic breakdown
Type | Count | As % |
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Scientists | 14 | 50% |
Members of the public | 11 | 39% |
Science communicators (journalists, bloggers, editors) | 2 | 7% |
Practitioners (doctors, other healthcare professionals) | 1 | 4% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 4% |
Indonesia | 1 | 2% |
Norway | 1 | 2% |
Unknown | 52 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 11 | 20% |
Student > Ph. D. Student | 10 | 18% |
Student > Master | 7 | 13% |
Professor > Associate Professor | 5 | 9% |
Other | 4 | 7% |
Other | 7 | 13% |
Unknown | 12 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 22 | 39% |
Biochemistry, Genetics and Molecular Biology | 9 | 16% |
Computer Science | 3 | 5% |
Medicine and Dentistry | 2 | 4% |
Immunology and Microbiology | 2 | 4% |
Other | 5 | 9% |
Unknown | 13 | 23% |