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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
|
|---|---|
| Published in |
Plant Methods, September 2014
|
| DOI | 10.1186/1746-4811-10-29 |
| Pubmed ID | |
| Authors |
Asmini Athman, Sandra K Tanz, Vanessa M Conn, Charlotte Jordans, Gwenda M Mayo, Weng W Ng, Rachel A Burton, Simon J Conn, Matthew Gilliham |
| Abstract |
An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These methods, although frequently used, can have limitations including their time-consuming nature, limited specificity, reliance upon well-annotated promoters, high cost, and the need for specialized equipment. In situ PCR is a relatively simple and rapid method that involves the amplification of specific mRNA directly within plant tissue whilst incorporating labelled nucleotides that are subsequently detected by immunohistochemistry. Another notable advantage of this technique is that it can be used on plants that are not easily genetically transformed. |
X Demographics
The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | 38% |
| Australia | 3 | 38% |
| Unknown | 2 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 50% |
| Scientists | 3 | 38% |
| Science communicators (journalists, bloggers, editors) | 1 | 13% |
Mendeley readers
The data shown below were compiled from readership statistics for 177 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 2 | 1% |
| Switzerland | 1 | <1% |
| Chile | 1 | <1% |
| Norway | 1 | <1% |
| Brazil | 1 | <1% |
| Sweden | 1 | <1% |
| India | 1 | <1% |
| Argentina | 1 | <1% |
| Spain | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 167 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 41 | 23% |
| Student > Ph. D. Student | 28 | 16% |
| Student > Master | 23 | 13% |
| Student > Bachelor | 17 | 10% |
| Student > Doctoral Student | 12 | 7% |
| Other | 26 | 15% |
| Unknown | 30 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 97 | 55% |
| Biochemistry, Genetics and Molecular Biology | 30 | 17% |
| Environmental Science | 4 | 2% |
| Immunology and Microbiology | 2 | 1% |
| Medicine and Dentistry | 2 | 1% |
| Other | 9 | 5% |
| Unknown | 33 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 May 2023.
All research outputs
#4,459,633
of 23,862,416 outputs
Outputs from Plant Methods
#266
of 1,137 outputs
Outputs of similar age
#46,614
of 252,871 outputs
Outputs of similar age from Plant Methods
#1
of 8 outputs
Altmetric has tracked 23,862,416 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,137 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 252,871 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them