Title |
Dysregulation of genome-wide gene expression and DNA methylation in abnormal cloned piglets
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Published in |
BMC Genomics, September 2014
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DOI | 10.1186/1471-2164-15-811 |
Pubmed ID | |
Authors |
Guanglei Li, Qitao Jia, Jianguo Zhao, Xinyun Li, Mei Yu, Melissa S Samuel, Shuhong Zhao, Randall S Prather, Changchun Li |
Abstract |
Epigenetic modifications (especially altered DNA methylation) resulting in altered gene expression may be one reason for development failure or abnormalities in cloned animals, but the underlying mechanism of the abnormal phenotype in cloned piglets remains unknown. Some cloned piglets in our study showed abnormal phenotypes such as large tongue (longer and thicker), weak muscles, and exomphalos. Here we conducted DNA methylation (DNAm) immunoprecipitation and high throughput sequencing (MeDIP-seq) and RNA sequencing (RNA-seq) of muscle tissues of cloned piglets to investigate the relationship of abnormal DNAm with gene dysregulation and the unusual phenotypes in cloned piglets. |
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