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Elucidation of the outer membrane proteome of Salmonella enterica serovar Typhimurium utilising a lipid-based protein immobilization technique

Overview of attention for article published in BMC Microbiology, January 2010
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (71st percentile)

Mentioned by

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3 tweeters

Citations

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20 Dimensions

Readers on

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59 Mendeley
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Title
Elucidation of the outer membrane proteome of Salmonella enterica serovar Typhimurium utilising a lipid-based protein immobilization technique
Published in
BMC Microbiology, January 2010
DOI 10.1186/1471-2180-10-44
Pubmed ID
Authors

Darren Chooneea, Roger Karlsson, Vesela Encheva, Cath Arnold, Hazel Appleton, Haroun Shah

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major cause of human gastroenteritis worldwide. The outer membrane proteins expressed by S. Typhimurium mediate the process of adhesion and internalisation within the intestinal epithelium of the host thus influencing the progression of disease. Since the outer membrane proteins are surface-exposed, they provide attractive targets for the development of improved antimicrobial agents and vaccines. Various techniques have been developed for their characterisation, but issues such as carryover of cytosolic proteins still remain a problem. In this study we attempted to characterise the surface proteome of S. Typhimurium using Lipid-based Protein Immobilisation technology in the form of LPI FlowCells. No detergents are required and no sample clean up is needed prior to downstream analysis. The immobilised proteins can be digested with proteases in multiple steps to increase sequence coverage, and the peptides eluted can be characterised directly by liquid chromatography - tandem mass spectrometry (LC-MS/MS) and identified from mass spectral database searches.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 3%
Uruguay 1 2%
Unknown 56 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 25%
Professor > Associate Professor 9 15%
Student > Ph. D. Student 8 14%
Student > Master 7 12%
Student > Bachelor 5 8%
Other 11 19%
Unknown 4 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 47%
Biochemistry, Genetics and Molecular Biology 5 8%
Medicine and Dentistry 4 7%
Immunology and Microbiology 4 7%
Environmental Science 3 5%
Other 10 17%
Unknown 5 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 September 2014.
All research outputs
#2,317,288
of 5,036,385 outputs
Outputs from BMC Microbiology
#416
of 1,012 outputs
Outputs of similar age
#58,651
of 137,475 outputs
Outputs of similar age from BMC Microbiology
#9
of 35 outputs
Altmetric has tracked 5,036,385 research outputs across all sources so far. This one has received more attention than most of these and is in the 51st percentile.
So far Altmetric has tracked 1,012 research outputs from this source. They receive a mean Attention Score of 2.8. This one has gotten more attention than average, scoring higher than 55% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 137,475 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.