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Amplicon sequencing of bacterial microbiota in abortion material from cattle

Overview of attention for article published in Veterinary Research, October 2017
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  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

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1 blog
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34 Mendeley
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Title
Amplicon sequencing of bacterial microbiota in abortion material from cattle
Published in
Veterinary Research, October 2017
DOI 10.1186/s13567-017-0470-1
Pubmed ID
Authors

Sara Vidal, Kristel Kegler, Horst Posthaus, Vincent Perreten, Sabrina Rodriguez-Campos

Abstract

Abortions in cattle have a significant economic impact on animal husbandry and require prompt diagnosis for surveillance of epizootic infectious agents. Since most abortions are not epizootic but sporadic with often undetected etiologies, this study examined the bacterial community present in the placenta (PL, n = 32) and fetal abomasal content (AC, n = 49) in 64 cases of bovine abortion by next generation sequencing (NGS) of the 16S rRNA gene. The PL and AC from three fetuses of dams that died from non-infectious reasons were included as controls. All samples were analyzed by bacterial culture, and 17 were examined by histopathology. We observed 922 OTUs overall and 267 taxa at the genus level. No detectable bacterial DNA was present in the control samples. The microbial profiles of the PL and AC differed significantly, both in their composition (PERMANOVA), species richness and Chao-1 (Mann-Whitney test). In both organs, Pseudomonas was the most abundant genus. The combination of NGS and culture identified opportunistic pathogens of interest in placentas with lesions, such as Vibrio metschnikovii, Streptococcus uberis, Lactococcus lactis and Escherichia coli. In placentas with lesions where culturing was unsuccessful, Pseudomonas and unidentified Aeromonadaceae were identified by NGS displaying high number of reads. Three cases with multiple possible etiologies and placentas presenting lesions were detected by NGS. Amplicon sequencing has the potential to uncover unknown etiological agents. These new insights on cattle abortion extend our focus to previously understudied opportunistic abortive bacteria.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 34 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 21%
Other 3 9%
Student > Bachelor 3 9%
Student > Master 3 9%
Student > Ph. D. Student 3 9%
Other 4 12%
Unknown 11 32%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 7 21%
Biochemistry, Genetics and Molecular Biology 4 12%
Medicine and Dentistry 4 12%
Agricultural and Biological Sciences 2 6%
Immunology and Microbiology 2 6%
Other 2 6%
Unknown 13 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 9. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2017.
All research outputs
#4,169,998
of 25,382,440 outputs
Outputs from Veterinary Research
#186
of 1,337 outputs
Outputs of similar age
#69,721
of 333,631 outputs
Outputs of similar age from Veterinary Research
#6
of 30 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,337 research outputs from this source. They receive a mean Attention Score of 5.0. This one has done well, scoring higher than 86% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 333,631 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 30 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.