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Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases

Overview of attention for article published in Genome Biology, October 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (91st percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

news
2 news outlets
twitter
8 X users
patent
2 patents

Citations

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105 Dimensions

Readers on

mendeley
146 Mendeley
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Title
Perfectly matched 20-nucleotide guide RNA sequences enable robust genome editing using high-fidelity SpCas9 nucleases
Published in
Genome Biology, October 2017
DOI 10.1186/s13059-017-1325-9
Pubmed ID
Authors

Dingbo Zhang, Huawei Zhang, Tingdong Li, Kunling Chen, Jin-Long Qiu, Caixia Gao

Abstract

High-fidelity SpCas9 variants (eSpCas9 and SpCas9-HF1) have been engineered to reduce off-target effects. We found that changes in guide RNA length induced significant reductions in the editing activities of SpCas9 variants in plant cells. Single guide RNAs harboring precise, perfectly matched 20-nucleotide guide sequences are necessary for high on-target editing activities of eSpCas9 and SpCas9-HF1. Precise 20-nucleotide guide sequences derived from tRNA-sgRNA precursors enable robust on-target editing by these variants with enhanced specificity. Our work reveals an effective way of enhancing the use of the high-fidelity SpCas9 nucleases for efficient and precise genome engineering.

X Demographics

X Demographics

The data shown below were collected from the profiles of 8 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 146 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 146 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 33 23%
Researcher 25 17%
Student > Master 21 14%
Student > Bachelor 14 10%
Student > Doctoral Student 7 5%
Other 13 9%
Unknown 33 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 49 34%
Agricultural and Biological Sciences 47 32%
Medicine and Dentistry 3 2%
Engineering 2 1%
Physics and Astronomy 2 1%
Other 6 4%
Unknown 37 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 28. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 October 2023.
All research outputs
#1,385,597
of 25,382,440 outputs
Outputs from Genome Biology
#1,089
of 4,468 outputs
Outputs of similar age
#27,943
of 333,588 outputs
Outputs of similar age from Genome Biology
#31
of 62 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,468 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 27.6. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 333,588 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 91% of its contemporaries.
We're also able to compare this research output to 62 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.