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A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid-resistant Aedes aegypti

Overview of attention for article published in Parasites & Vectors, October 2017
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Title
A multiplex PCR for detection of knockdown resistance mutations, V1016G and F1534C, in pyrethroid-resistant Aedes aegypti
Published in
Parasites & Vectors, October 2017
DOI 10.1186/s13071-017-2416-x
Pubmed ID
Authors

Jassada Saingamsook, Atiporn Saeung, Jintana Yanola, Nongkran Lumjuan, Catherine Walton, Pradya Somboon

Abstract

Mutation of the voltage-gated sodium channel (VGSC) gene, or knockdown resistance (kdr) gene, is an important resistance mechanism of the dengue vector Aedes aegypti mosquitoes against pyrethroids. In many countries in Asia, a valine to glycine substitution (V1016G) and a phenylalanine to cysteine substitution (F1534C) are common in Ae. aegypti populations. The G1016 and C1534 allele frequencies have been increasing in recent years, and hence there is a need to have a simple and inexpensive tool to monitor the alleles in large scale. A multiplex PCR to detect V1016G and F1534C mutations has been developed in the current study. This study utilized primers from previous studies for detecting the mutation at position 1016 and newly designed primers to detect variants at position 1534. The PCR conditions were validated and compared with DNA sequencing using known kdr mutant laboratory strains and field collected mosquitoes. The efficacy of this method was also compared with allele-specific PCR (AS-PCR). The results of our multiplex PCR were in complete agreement with sequencing data and better than the AS-PCR. In addition, the efficiency of two non-toxic DNA staining dyes, Ultrapower™ and RedSafe™, were evaluated by comparing with ethidium bromide (EtBr) and the results were satisfactory. Our multiplex PCR method is highly reliable and useful for implementing vector surveillance in locations where the two alleles co-occur.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 100 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 100 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 21 21%
Researcher 14 14%
Student > Master 13 13%
Student > Postgraduate 9 9%
Student > Ph. D. Student 7 7%
Other 16 16%
Unknown 20 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 27 27%
Agricultural and Biological Sciences 23 23%
Medicine and Dentistry 9 9%
Environmental Science 6 6%
Immunology and Microbiology 3 3%
Other 9 9%
Unknown 23 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#20,449,496
of 23,005,189 outputs
Outputs from Parasites & Vectors
#4,877
of 5,499 outputs
Outputs of similar age
#282,910
of 324,392 outputs
Outputs of similar age from Parasites & Vectors
#140
of 164 outputs
Altmetric has tracked 23,005,189 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,499 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 164 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.