Title |
ORF1a of highly pathogenic PRRS attenuated vaccine virus plays a key role in neutralizing antibody induction in piglets and virus neutralization in vitro
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Published in |
Virology Journal, August 2017
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DOI | 10.1186/s12985-017-0825-2 |
Pubmed ID | |
Authors |
Chaoliang Leng, Wuchao Zhang, Hongliang Zhang, Yunchao Kan, Lunguang Yao, Hongyue Zhai, Mingliang Li, Zhen Li, Chunxiao Liu, Tongqing An, Jinmei Peng, Qian Wang, Yumin Leng, Xuehui Cai, Zhijun Tian, Guangzhi Tong |
Abstract |
Currently, porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important viral pathogens in swine in most countries, especially China. Two PRRSV attenuated live vaccine strains (HuN4-F112 and CH-1R) are currently widely used in China. Our previous study showed that HuN4-F112, but not CH-1R, induced high anti-nucleocapsid (N) antibody and neutralizing antibody (NA) titers. Additionally, sera from HuN4-F112 inoculated pigs induced low cross neutralization of CH-1R. In the present study, 6 chimeric viruses through exchanging 5' untranslated region (UTR) + open reading frame (ORF)1a, ORF1b, and ORF2-7 + 3'UTR between HuN4-F112 and CH-1R were constructed and rescued based on the infectious clones of rHuN4-F112 and rCH-1R. The characteristics of these viruses were investigated in vitro and vivo. All the three fragments, 5'UTR + ORF1a, ORF1b, and ORF2-7 + 3'UTR, could affect the replication efficiencies of rHuN4-F112 and rCH-1R in vitro. Additionally, both 5'UTR + ORF1a and ORF2-7 + 3'UTR affected the anti-N antibody and NA responses targeting rHuN4-F112 and rCH-1R in piglets. The 5'UTR + ORF1a region of HuN4-F112 played a key role in inducing NAs in piglets. Furthermore, we confirmed for the first time that ORF1a contains a neutralization region. This study provides important information that can be used for further study of the generation of anti-PRRSV NAs. |
X Demographics
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Unknown | 4 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 75% |
Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
Geographical breakdown
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Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 6 | 26% |
Student > Ph. D. Student | 6 | 26% |
Student > Bachelor | 2 | 9% |
Professor | 2 | 9% |
Student > Master | 2 | 9% |
Other | 3 | 13% |
Unknown | 2 | 9% |
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Veterinary Science and Veterinary Medicine | 5 | 22% |
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Pharmacology, Toxicology and Pharmaceutical Science | 3 | 13% |
Agricultural and Biological Sciences | 3 | 13% |
Computer Science | 1 | 4% |
Other | 3 | 13% |
Unknown | 4 | 17% |