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Fibroblast Growth Factor-2 alone as an efficient inducer for differentiation of human bone marrow mesenchymal stem cells into dopaminergic neurons

Overview of attention for article published in Journal of Biomedical Science, September 2014
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Title
Fibroblast Growth Factor-2 alone as an efficient inducer for differentiation of human bone marrow mesenchymal stem cells into dopaminergic neurons
Published in
Journal of Biomedical Science, September 2014
DOI 10.1186/s12929-014-0083-1
Pubmed ID
Authors

Sushmita Bose Nandy, Sujata Mohanty, Manisha Singh, Madhuri Behari, Balram Airan

Abstract

BackgroundThe reported efficiency of differentiation of human bone marrow derived Mesenchymal Stem Cells (hBM-MSC) into dopaminergic neurons with different inducers is found to vary. Thus, in the current study we have investigated the response of hBM MSCs to some of the neuronal inducers and their combinations. Neuronal differentiation inducing agents Fibroblastic Growth Factor 2 (FGF2), Sonic Hedge Hog (Shh), Fibroblastic Growth Factor 8 (FGF8) & All Trans Retinoic Acid (ATRA) were used either singly or in varied combinations.ResultsThe differentiated and undifferentiated hBM MSC were characterized in terms of morphology, expression of cell markers at transcriptional and translational levels, amount of dopamine secreted by the cells in the media and changes in cell membrane potential by calcium ions imaging. Induced hBM MSC revealed neuron like morphology and expressed cellular markers suggesting neuronal differentiation with all the inducing agents. However, upon quantitative analysis through qPCR, cells induced with FGF2 were found to show maximum expression of tyrosine hydroxylase (TH) by 47.5 folds. Immunofluorescence analysis of differentiated and undifferentiated cells also revealed expression of nestin, neurofilament, microtubule associated protein- 2, beta tubulin III and TH in differentiated cells, at translational level. This data was supported by immunoblotting analysis. Further, ELISA study also supported the release of dopamine by cultures induced with FGF2. When the cells were depolarised with KCl solution, those induced with Shh & FGF8 showed maximum calcium ion trafficking, followed by the cells induced with FGF2 only.ConclusionsWe conclude that hBM MSCs can be coaxed to differentiate efficiently into dopaminergic neurons in the presence of a very simple media cocktail containing only one main inducer like FGF2 and thus contribute towards cellular therapy in Parkinson's and other related disorders. These dopaminergic neurons are also functionally active, as shown by calcium ion trafficking.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Poland 1 1%
France 1 1%
Unknown 84 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 15 17%
Researcher 14 16%
Student > Master 14 16%
Other 6 7%
Student > Bachelor 5 6%
Other 19 22%
Unknown 13 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 20%
Biochemistry, Genetics and Molecular Biology 15 17%
Neuroscience 11 13%
Medicine and Dentistry 9 10%
Nursing and Health Professions 3 3%
Other 14 16%
Unknown 17 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 October 2014.
All research outputs
#20,657,128
of 25,374,917 outputs
Outputs from Journal of Biomedical Science
#871
of 1,101 outputs
Outputs of similar age
#192,531
of 263,254 outputs
Outputs of similar age from Journal of Biomedical Science
#9
of 12 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,101 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 11.0. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
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We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one is in the 25th percentile – i.e., 25% of its contemporaries scored the same or lower than it.