Title |
Molecular surveillance of pfhrp2 and pfhrp3 deletions in Plasmodium falciparum isolates from Mozambique
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Published in |
Malaria Journal, October 2017
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DOI | 10.1186/s12936-017-2061-z |
Pubmed ID | |
Authors |
Himanshu Gupta, Gloria Matambisso, Beatriz Galatas, Pau Cisteró, Lidia Nhamussua, Wilson Simone, Jane Cunningham, N. Regina Rabinovich, Pedro Alonso, Francisco Saute, Pedro Aide, Alfredo Mayor |
Abstract |
Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. Plasmodium falciparum isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates from individuals who were negative by RDT but positive by microscopy and/or qPCR (n = 69) using gene-specific PCRs, with kelch13 PCR as the parasite DNA control. Lack of pfhrp2 PCR amplification was observed in one of the 69 isolates subjected to molecular analysis [1.45% (95% CI 0.3-7.8%)]. The low prevalence of pfhrp2 deletions suggests that RDTs will detect the vast majority of the P. falciparum infections. Nevertheless, active surveillance for changing deletion frequencies is required. |
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