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AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation

Overview of attention for article published in BMC Biotechnology, October 2014
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#40 of 502)
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (81st percentile)

Mentioned by

news
1 news outlet
twitter
2 tweeters

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
16 Mendeley
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Title
AAV2-mediated follistatin overexpression induces ovine primary myoblasts proliferation
Published in
BMC Biotechnology, October 2014
DOI 10.1186/s12896-014-0087-7
Pubmed ID
Authors

Mahmood Nazari, Fatemeh Salabi, Li Zhang, Fuping Zhao, Caihong Wei, Lixin Du

Abstract

BackgroundFollistatin (FST) has been shown to bind to some TGF-ß family members and can function as a potent myostatin (MSTN) antagonist. Recent studies have revealed that over-expression of FST by adeno-associated viruses increases muscle growth in mice, humans and nonhuman primates. In the present study, to determine the effect of FST on ovine primary myoblast (OPM) proliferation, FST was over-expressed using an adeno-associated virus serotype 2 (AAV 2) vector.ResultsWestern blot results showed that AAV induced the expression of FST protein in transduced OPM cells. Real-time quantitative PCR results indicated that over-expression of FST resulted in a dramatic increase in Akt I and CDK2 expression and a decrease in p21 expression. Moreover, cell cycle analysis confirmed that FST down-regulated p21, a CDK inhibitor, and increased the level of CDK2 expression in OPM cells. Hence, follistatin positively regulated the G1 to S progression. Our results showed that FST induced proliferation through a down-regulation of p21, as only the p21 expression level was down-regulated as a result of FST over-expression in myoblasts, whereas no change was observed in the level of p57 expression.ConclusionsThese results expanded our understanding of the regulation mechanism of FST in ovine primary myoblasts. Our results provide the first evidence that the AAV viral system can be used for gene transfer in ovine myoblast cells. Moreover, the results showed that an AAV vector can successfully induce the expression of FST in OPM cells in vitro. These findings demonstrated that FST over-expression induces proliferation through a down-regulation of the p21 gene under proliferating conditions.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 25%
Student > Ph. D. Student 3 19%
Professor > Associate Professor 2 13%
Researcher 2 13%
Student > Bachelor 1 6%
Other 1 6%
Unknown 3 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 44%
Biochemistry, Genetics and Molecular Biology 3 19%
Neuroscience 2 13%
Unspecified 1 6%
Unknown 3 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 October 2015.
All research outputs
#876,699
of 8,701,761 outputs
Outputs from BMC Biotechnology
#40
of 502 outputs
Outputs of similar age
#24,668
of 203,197 outputs
Outputs of similar age from BMC Biotechnology
#3
of 16 outputs
Altmetric has tracked 8,701,761 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 502 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 203,197 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.