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Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil

Overview of attention for article published in Parasites & Vectors, October 2017
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86 Mendeley
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Title
Distribution of Blastocystis subtypes isolated from humans from an urban community in Rio de Janeiro, Brazil
Published in
Parasites & Vectors, October 2017
DOI 10.1186/s13071-017-2458-0
Pubmed ID
Authors

Carolina Valença Barbosa, Rosemary de Jesus Batista, Ricardo Pereira Igreja, Claudia Masini d’Avila Levy, Heloisa Werneck de Macedo, Helena Lúcia Carneiro Santos

Abstract

Blastocystis is a cosmopolitan protist parasite found in the human gastrointestinal tract and is highly prevalent in developing countries. Recent molecular studies have revealed extensive genetic diversity, which has been classified into different subtypes (STs) based on sequence analysis of small subunit ribosomal RNA gene. Blastocystis is one of the most common fecal parasites in Brazil, but the diversity of subtypes remains unknown in the country. This study aimed to determine the distribution of Blastocystis STs in an urban community in Duque de Caxias, Rio de Janeiro, Brazil. A total of 64 stool samples positive for Blastocystis in Pavlova's medium were subtyped by PCR and sequenced using primers targeting the small subunit rRNA gene, in addition to phylogenetic analysis and subtype-specific PCR using sequence-tagged-site (STS) primers. Endolimax nana (14%), Entamoeba complex (10.5%), Taenia sp. (0.6%), Trichuris trichiura (1.3%) and Enterobius vermicularis (1.3%) were detected in Blastocystis-positive samples. Of the 64 samples tested by PCR/DNA sequencing, 55 were identified as ST1 (42%), ST3 (49%), ST2 (7%) and ST4 (2%), and the presence of mixed ST (ST1 + ST3) infection was detected in nine samples (14%). DNA sequencing and phylogenetic analysis of Brazilian Blastocystis isolates identified four different subtypes. To our knowledge, this study provided the first genetic characterization of Blastocystis subtypes in an urban area of Rio de Janeiro, Brazil. We also identified ST4 for the first time in Brazil. Further studies are necessary to determine the distribution of STs across human populations in Rio de Janeiro.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 86 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 12 14%
Researcher 8 9%
Other 7 8%
Student > Bachelor 7 8%
Student > Ph. D. Student 7 8%
Other 12 14%
Unknown 33 38%
Readers by discipline Count As %
Medicine and Dentistry 9 10%
Immunology and Microbiology 7 8%
Agricultural and Biological Sciences 6 7%
Biochemistry, Genetics and Molecular Biology 6 7%
Nursing and Health Professions 4 5%
Other 14 16%
Unknown 40 47%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 November 2017.
All research outputs
#14,957,541
of 23,006,268 outputs
Outputs from Parasites & Vectors
#3,109
of 5,502 outputs
Outputs of similar age
#193,961
of 327,865 outputs
Outputs of similar age from Parasites & Vectors
#91
of 165 outputs
Altmetric has tracked 23,006,268 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,502 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 327,865 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 165 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.