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Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting

Overview of attention for article published in BMC Infectious Diseases, October 2014
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Title
Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting
Published in
BMC Infectious Diseases, October 2014
DOI 10.1186/s12879-014-0558-4
Pubmed ID
Authors

Maria Cristina Carvalho Espírito-Santo, Mónica Viviana Alvarado-Mora, Emmanuel Dias-Neto, Lívia Souza Botelho-Lima, João Paulo Moreira, Maria Amorim, Pedro Luiz Silva Pinto, Ashley R Heath, Vera Lúcia Pagliusi Castilho, Elenice Messias do Nascimento Gonçalves, Expedito José de Albuquerque Luna, Flair José Carrilho, João Renato Rebello Pinho, Ronaldo Cesar Borges Gryschek

Abstract

Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, schistosomiasis has been reported in 19 states, showing areas of high and medium endemicity and a wide range of areas of low endemicity (ALE). Barra Mansa in Rio de Janeiro state has an estimated prevalence of 1%. ALE represent a new challenge for the helminth control because about 75% of infected individuals are asymptomatic and infections occur with a low parasite load (<100 eggs per gram of feces), causing a decrease in sensitivity of stool parasitological techniques, which are a reference for the laboratory diagnosis of this helminth. The objective of this study was to evaluate the performance of a TaqMan quantitative polymerase chain reaction (qPCR) technique in serum and feces DNA samples using the techniques of Kato-Katz (KK), Hoffman, Pons and Janer (HH) as references, during an epidemiological survey using fecal samples and sera from randomized residents from an ALE.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Burkina Faso 1 2%
Brazil 1 2%
Unknown 57 97%

Demographic breakdown

Readers by professional status Count As %
Student > Master 11 19%
Student > Bachelor 9 15%
Student > Ph. D. Student 6 10%
Librarian 5 8%
Researcher 4 7%
Other 9 15%
Unknown 15 25%
Readers by discipline Count As %
Medicine and Dentistry 16 27%
Biochemistry, Genetics and Molecular Biology 12 20%
Agricultural and Biological Sciences 6 10%
Nursing and Health Professions 4 7%
Pharmacology, Toxicology and Pharmaceutical Science 1 2%
Other 5 8%
Unknown 15 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 October 2014.
All research outputs
#20,241,019
of 22,768,097 outputs
Outputs from BMC Infectious Diseases
#6,459
of 7,668 outputs
Outputs of similar age
#217,181
of 260,450 outputs
Outputs of similar age from BMC Infectious Diseases
#153
of 185 outputs
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