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Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress

Overview of attention for article published in BMC Plant Biology, October 2017
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Title
Genome-wide analysis of wheat calcium ATPases and potential role of selected ACAs and ECAs in calcium stress
Published in
BMC Plant Biology, October 2017
DOI 10.1186/s12870-017-1112-5
Pubmed ID
Authors

Roohi Aslam, Lorraine E. Williams, Muhammad Faraz Bhatti, Nasar Virk

Abstract

P2- type calcium ATPases (ACAs-auto inhibited calcium ATPases and ECAs-endoplasmic reticulum calcium ATPases) belong to the P- type ATPase family of active membrane transporters and are significantly involved in maintaining accurate levels of Ca(2+), Mn(2+) and Zn(2+) in the cytosol as well as playing a very important role in stress signaling, stomatal opening and closing and pollen tube growth. Here we report the identification and possible role of some of these ATPases from wheat. In this study, ACA and ECA sequences of six species (belonging to Poaceae) were retrieved from different databases and a phylogenetic tree was constructed. A high degree of evolutionary relatedness was observed among P2 sequences characterized in this study. Members of the respective groups from different plant species were observed to fall under the same clade. This pattern highlights the common ancestry of P2- type calcium ATPases. Furthermore, qRT-PCR was used to analyse the expression of selected ACAs and ECAs from Triticum aestivum (wheat) under calcium toxicity and calcium deficiency. The data indicated that expression of ECAs is enhanced under calcium stress, suggesting possible roles of these ATPases in calcium homeostasis in wheat. Similarly, the expression of ACAs was significantly different in plants grown under calcium stress as compared to plants grown under control conditions. This gives clues to the role of ACAs in signal transduction during calcium stress in wheat. Here we concluded that wheat genome consists of nine P2B and three P2A -type calcium ATPases. Moreover, gene loss events in wheat ancestors lead to the loss of a particular homoeolog of a gene in wheat. To elaborate the role of these wheat ATPases, qRT-PCR was performed. The results indicated that when plants are exposed to calcium stress, both P2A and P2B gene expression get enhanced. This further gives clues about the possible role of these ATPases in wheat in calcium management. These findings can be useful in future for genetic manipulations as well as in wheat genome annotation process.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 29%
Professor > Associate Professor 2 12%
Student > Ph. D. Student 2 12%
Researcher 2 12%
Student > Doctoral Student 1 6%
Other 2 12%
Unknown 3 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 10 59%
Biochemistry, Genetics and Molecular Biology 4 24%
Unknown 3 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 June 2018.
All research outputs
#10,403,228
of 13,046,126 outputs
Outputs from BMC Plant Biology
#1,132
of 1,705 outputs
Outputs of similar age
#227,510
of 310,650 outputs
Outputs of similar age from BMC Plant Biology
#104
of 173 outputs
Altmetric has tracked 13,046,126 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,705 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 20th percentile – i.e., 20% of its peers scored the same or lower than it.
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We're also able to compare this research output to 173 others from the same source and published within six weeks on either side of this one. This one is in the 24th percentile – i.e., 24% of its contemporaries scored the same or lower than it.