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Protein corona: implications for nanoparticle interactions with pulmonary cells

Overview of attention for article published in Particle and Fibre Toxicology, October 2017
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (64th percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

Mentioned by

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5 tweeters
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1 Facebook page

Citations

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70 Dimensions

Readers on

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88 Mendeley
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Title
Protein corona: implications for nanoparticle interactions with pulmonary cells
Published in
Particle and Fibre Toxicology, October 2017
DOI 10.1186/s12989-017-0223-3
Pubmed ID
Authors

Nagarjun V. Konduru, Ramon M. Molina, Archana Swami, Flavia Damiani, Georgios Pyrgiotakis, Paulo Lin, Patrizia Andreozzi, Thomas C. Donaghey, Philip Demokritou, Silke Krol, Wolfgang Kreyling, Joseph D. Brain

Abstract

We previously showed that cerium oxide (CeO2), barium sulfate (BaSO4) and zinc oxide (ZnO) nanoparticles (NPs) exhibited different lung toxicity and pulmonary clearance in rats. We hypothesize that these NPs acquire coronas with different protein compositions that may influence their clearance from the lungs. CeO2, silica-coated CeO2, BaSO4, and ZnO NPs were incubated in rat lung lining fluid in vitro. Then, gel electrophoresis followed by quantitative mass spectrometry was used to characterize the adsorbed proteins stripped from these NPs. We also measured uptake of instilled NPs by alveolar macrophages (AMs) in rat lungs using electron microscopy. Finally, we tested whether coating of gold NPs with albumin would alter their lung clearance in rats. We found that the amounts of nine proteins in the coronas formed on the four NPs varied significantly. The amounts of albumin, transferrin and α-1 antitrypsin were greater in the coronas of BaSO4 and ZnO than that of the two CeO2 NPs. The uptake of BaSO4 in AMs was less than CeO2 and silica-coated CeO2 NPs. No identifiable ZnO NPs were observed in AMs. Gold NPs coated with albumin or citrate instilled into the lungs of rats acquired the similar protein coronas and were cleared from the lungs to the same extent. We show that different NPs variably adsorb proteins from the lung lining fluid. The amount of albumin in the NP corona varies as does NP uptake by AMs. However, albumin coating does not affect the translocation of gold NPs across the air-blood barrier. A more extensive database of corona composition of a diverse NP library will develop a platform to help predict the effects and biokinetics of inhaled NPs.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 88 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 88 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 21 24%
Student > Ph. D. Student 17 19%
Student > Master 14 16%
Student > Bachelor 11 13%
Student > Doctoral Student 6 7%
Other 10 11%
Unknown 9 10%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 13 15%
Chemistry 12 14%
Engineering 7 8%
Agricultural and Biological Sciences 7 8%
Biochemistry, Genetics and Molecular Biology 7 8%
Other 25 28%
Unknown 17 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 June 2020.
All research outputs
#5,589,468
of 17,913,039 outputs
Outputs from Particle and Fibre Toxicology
#183
of 503 outputs
Outputs of similar age
#117,486
of 330,554 outputs
Outputs of similar age from Particle and Fibre Toxicology
#15
of 55 outputs
Altmetric has tracked 17,913,039 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 503 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.8. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 330,554 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.
We're also able to compare this research output to 55 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.