Title |
pEPito: a significantly improved non-viral episomal expression vector for mammalian cells
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Published in |
BMC Biotechnology, March 2010
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DOI | 10.1186/1472-6750-10-20 |
Pubmed ID | |
Authors |
Rudolf Haase, Orestis Argyros, Suet-Ping Wong, Richard P Harbottle, Hans J Lipps, Manfred Ogris, Terese Magnusson, Maria G Vizoso Pinto, Jürgen Haas, Armin Baiker |
Abstract |
The episomal replication of the prototype vector pEPI-1 depends on a transcription unit starting from the constitutively expressed Cytomegalovirus immediate early promoter (CMV-IEP) and directed into a 2000 bp long matrix attachment region sequence (MARS) derived from the human beta-interferon gene. The original pEPI-1 vector contains two mammalian transcription units and a total of 305 CpG islands, which are located predominantly within the vector elements necessary for bacterial propagation and known to be counterproductive for persistent long-term transgene expression. |
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Mendeley readers
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