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Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection

Overview of attention for article published in BMC Research Notes, March 2014
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Title
Sucrose stabilization of Respiratory Syncytial Virus (RSV) during nebulization and experimental infection
Published in
BMC Research Notes, March 2014
DOI 10.1186/1756-0500-7-158
Pubmed ID
Authors

Drew D Grosz, Albert van Geelen, Jack M Gallup, Shannon J Hostetter, Rachel J Derscheid, Mark R Ackermann

Abstract

Respiratory syncytial virus (RSV) is a common respiratory pathogen that can cause severe pneumonia. In vivo studies of RSV can be difficult due to variation in viral infection and disease severity in some animal models. Factors that may contribute to the variation are decreases in viral titer due to preparation and storage and method of virus administration. Nebulization is one method of RSV administration that provides even distribution of virus to all lung lobes; however, the exact quantity of the virus killed by nebulization is not defined. To test the hypothesis that sucrose enhances RSV stability and infectivity, a series of in vitro experiments were conducted with RSV strain Memphis 37 stored at varying concentrations (0%, 3%, 5%, 8%, 10%, 15%, and 20%) of sucrose as a possible cryo- and nebulization protectant. The optimal in vitro concentration was then assessed in vivo in a lamb model.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 39 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 13%
Student > Ph. D. Student 5 13%
Professor > Associate Professor 3 8%
Student > Master 3 8%
Student > Bachelor 2 5%
Other 4 10%
Unknown 17 44%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 13%
Agricultural and Biological Sciences 4 10%
Medicine and Dentistry 3 8%
Engineering 2 5%
Immunology and Microbiology 1 3%
Other 3 8%
Unknown 21 54%