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GnRH agonists induce endometrial epithelial cell apoptosis via GRP78 down-regulation

Overview of attention for article published in Journal of Translational Medicine, November 2014
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Title
GnRH agonists induce endometrial epithelial cell apoptosis via GRP78 down-regulation
Published in
Journal of Translational Medicine, November 2014
DOI 10.1186/s12967-014-0306-y
Pubmed ID
Authors

Huinan Weng, Fenghua Liu, Shuiwang Hu, Li, Yifeng Wang

Abstract

BackgroundEndometriosis is a benign chronic gynecological disease that affects women of reproductive age, characterized by the presence of functional endometrial tissues outside the uterine cavity. GnRH agonists exhibit anti-proliferative and apoptosis-enhancing activities and have long been used for the treatment of endometriosis. There is a critical need to identify the signaling modules involving GnRH agonist therapy for the treatment of endometriosis. In this study, we compared the proteomic profiles of endometriosis in patients before and after GnRH agonist therapy to identify proteins that might provide further information concerning the mechanisms underlying the functions of GnRH agonists.MethodsA total of 55 protein spots with different abundances were observed using Difference Gel Electrophoresis (DIGE), and 26 of these proteins were assigned clear identities through Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Tandem Mass Spectroscopy (MALDI-TOF/TOF MS).ResultsWe validated four of these proteins through Western blotting and immunohistochemistry using human endometrial tissue. We also characterized the effect of Leuprolide acetate (LA) on the apoptosis of eutopic endometrial epithelial cells. LA treatment significantly promoted the apoptosis of eutopic endometrial epithelial cells and inhibited the expression of the anti-apoptotic factor GRP78. GRP78 knockdown enhanced LA-induced cell apoptosis, whereas, the overexpression of GRP78 in eutopic endometrial epithelial cells suppresses LA-induced apoptosis.ConclusionThese results suggest that GnRH agonists induce endometrial epithelial cell apoptosis via GRP78 down-regulation. This study might provide an important molecular framework for further evaluation of GnRH agonist therapy.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 15%
Student > Doctoral Student 2 10%
Student > Bachelor 2 10%
Student > Postgraduate 2 10%
Student > Ph. D. Student 2 10%
Other 4 20%
Unknown 5 25%
Readers by discipline Count As %
Medicine and Dentistry 7 35%
Biochemistry, Genetics and Molecular Biology 2 10%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Agricultural and Biological Sciences 1 5%
Nursing and Health Professions 1 5%
Other 2 10%
Unknown 6 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 November 2014.
All research outputs
#20,242,136
of 22,769,322 outputs
Outputs from Journal of Translational Medicine
#3,305
of 3,982 outputs
Outputs of similar age
#218,767
of 262,191 outputs
Outputs of similar age from Journal of Translational Medicine
#72
of 97 outputs
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