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Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells

Overview of attention for article published in Stem Cell Research & Therapy, November 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

blogs
1 blog
twitter
2 X users
patent
3 patents

Citations

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27 Dimensions

Readers on

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29 Mendeley
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Title
Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells
Published in
Stem Cell Research & Therapy, November 2017
DOI 10.1186/s13287-017-0698-8
Pubmed ID
Authors

Linli Wang, Yuehua Chen, Chunyan Guan, Zhiju Zhao, Qiang Li, Jianguo Yang, Jian Mo, Bin Wang, Wei Wu, Xiaohui Yang, Libing Song, Jun Li

Abstract

Because the lack of an induced pluripotent stem cell (iPSC) induction system with optimal safety and efficiency limits the application of these cells, development of such a system is important. To create such an induction system, we screened a variety of reprogrammed plasmid combinations and multiple compounds and then verified the system's feasibility using urine cells from different individuals. We also compared large-scale iPSC chromosomal variations and expression of genes associated with genomic stability between this system and the traditional episomal system using karyotype and quantitative reverse transcription polymerase chain reaction analyses. We developed a high-efficiency episomal system, the 6F/BM1-4C system, lacking tumorigenic factors for human urine-derived cell (hUC) reprogramming. This system includes six low-risk factors (6F), Oct4, Glis1, Klf4, Sox2, L-Myc, and the miR-302 cluster. Transfected hUCs were treated with four compounds (4C), inhibitor of lysine-demethylase1, methyl ethyl ketone, glycogen synthase kinase 3 beta, and histone deacetylase, within a short time period. Comparative analysis revealed significantly decreased chromosomal variation in iPSCs and significantly increased Sirt1 expression compared with iPSCs induced using the traditional episomal system. The 6F/BM1-4C system effectively induces reprogramming of urine cells in samples obtained from different individuals. iPSCs induced using the 6F/BM1-4C system are more stable at the cytogenetic level and have potential value for clinical application.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 4 14%
Student > Ph. D. Student 4 14%
Student > Master 4 14%
Student > Bachelor 2 7%
Other 2 7%
Other 2 7%
Unknown 11 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 24%
Agricultural and Biological Sciences 5 17%
Medicine and Dentistry 3 10%
Social Sciences 1 3%
Nursing and Health Professions 1 3%
Other 2 7%
Unknown 10 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 June 2022.
All research outputs
#2,178,687
of 25,918,104 outputs
Outputs from Stem Cell Research & Therapy
#133
of 2,801 outputs
Outputs of similar age
#42,076
of 344,150 outputs
Outputs of similar age from Stem Cell Research & Therapy
#5
of 80 outputs
Altmetric has tracked 25,918,104 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,801 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has done particularly well, scoring higher than 95% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 344,150 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 80 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.