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Pancreatic cyst fluid harbors a unique microbiome

Overview of attention for article published in Microbiome, November 2017
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (86th percentile)

Mentioned by

blogs
1 blog
twitter
15 tweeters

Citations

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29 Dimensions

Readers on

mendeley
66 Mendeley
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Title
Pancreatic cyst fluid harbors a unique microbiome
Published in
Microbiome, November 2017
DOI 10.1186/s40168-017-0363-6
Pubmed ID
Authors

Shan Li, Gwenny M. Fuhler, Nahush BN, Tony Jose, Marco J. Bruno, Maikel P. Peppelenbosch, Sergey R. Konstantinov

Abstract

It is clear that specific intestinal bacteria are involved in the development of different premalignant conditions along the gastrointestinal tract. An analysis of the microbial constituents in the context of pancreatic cystic lesions has, however, as yet not been performed. This consideration prompted us to explore whether endoscopically obtained pancreatic cyst fluids (PCF) contain bacterial DNA and to determine the genera of bacteria present in such material. Total DNA was isolated from 69 PCF samples. Bacterial 16S rRNA gene-specific PCR was performed followed by Sanger sequencing and de novo deep sequencing for the V3-V4 variable region of 16S rRNA gene. We observed that 98.2% of the samples were positive in conventional PCR, and that 100% of selected PCF samples (n = 33) were positive for bacterial microbiota as determined by next generation sequencing (NGS). Comprehensive NGS data analysis of PCF showed the presence of 408 genera of bacteria, of which 17 bacterial genera were uniquely abundant to PCF, when compared to the Human Microbiome Project (HMP) database and 15 bacterial microbiota were uniquely abundant in HMP only. Bacteroides spp., Escherichia/Shigella spp., and Acidaminococcus spp. which were predominant in PCF, while also a substantial Staphylococcus spp. and Fusobacterium spp. component was detected. These results reveal and characterize an apparently specific bacterial ecosystem in pancreatic cyst fluid samples and may reflect the local microbiota in the pancreas. Some taxa with potential deleterious functions are present in the bacterial abundance profiles, suggesting that the unique microbiome in this specific niche may contribute to neoplastic processes in the pancreas. Further studies are needed to explore the intricate relationship between pathophysiological status in the host pancreas and its microbiota.

Twitter Demographics

The data shown below were collected from the profiles of 15 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 66 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 66 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 15 23%
Student > Ph. D. Student 12 18%
Other 6 9%
Student > Master 5 8%
Student > Bachelor 4 6%
Other 12 18%
Unknown 12 18%
Readers by discipline Count As %
Medicine and Dentistry 15 23%
Biochemistry, Genetics and Molecular Biology 11 17%
Agricultural and Biological Sciences 8 12%
Immunology and Microbiology 6 9%
Computer Science 2 3%
Other 4 6%
Unknown 20 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 15. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 April 2018.
All research outputs
#1,736,943
of 19,140,651 outputs
Outputs from Microbiome
#684
of 1,154 outputs
Outputs of similar age
#46,000
of 334,707 outputs
Outputs of similar age from Microbiome
#106
of 148 outputs
Altmetric has tracked 19,140,651 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,154 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 40.2. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 334,707 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 86% of its contemporaries.
We're also able to compare this research output to 148 others from the same source and published within six weeks on either side of this one. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.