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MiR-20a-5p represses the multi-drug resistance of osteosarcoma by targeting the SDC2 gene

Overview of attention for article published in Cancer Cell International, November 2017
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1 tweeter

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Title
MiR-20a-5p represses the multi-drug resistance of osteosarcoma by targeting the SDC2 gene
Published in
Cancer Cell International, November 2017
DOI 10.1186/s12935-017-0470-2
Pubmed ID
Authors

Fangfang Zhao, Youguang Pu, Mingda Cui, Haiyan Wang, Shanbao Cai

Abstract

As one of the hallmarks of cancer, chemoresistance hinders curative cancer chemotherapy in osteosarcoma (OS). MicroRNAs (miRNAs) act as key regulators of gene expression in diverse biological processes including the multi-chemoresistance of cancers. Based on the CCK8 experiments, we performed an RNA-seq-based miR-omic analysis of osteosarcoma (OS) cells (a multi-chemosensitive OS cell line G-292 and a multi-chemoresistant OS cell line SJSA-1) to detect the levels of miR-20a-5p. We predicted Homo sapiens syndecan 2 (SDC2) as one of the target genes of miR-20a-5p via several websites, which was further validated by detecting their expression of both mRNA and protein level in both the miR-20a-5p-mimic transfected G-292 and miR-20a-5p-antagomiR transfected SJSA-1 cells. The involvement of SDC2 with OS chemoresistance was checked by siRNA-mediated repression or overexpression of SDC2 gene. Cell viability was assessed by CCK8 assay. We found that the miR-20a-5p level was higher in G-292 cells than in SJSA-1 cells. Forced expression of miR-20a-5p counteracted OS chemoresistance in both cell culture and tumor xenografts in nude mice. As one of miR-20a-5p's targets, SDC2 was found to mediate the miR-20a-5p-induced repression of OS chemoresistance. Our results suggest that miR-20a-5p and SDC2 contribute to OS chemoresistance. The key players in the miR-20a-5p/SDC2 axis may be a potential diagnostic biomarker and therapeutic target for OS patients.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 38%
Lecturer > Senior Lecturer 1 13%
Student > Bachelor 1 13%
Lecturer 1 13%
Professor 1 13%
Other 1 13%
Readers by discipline Count As %
Medicine and Dentistry 3 38%
Biochemistry, Genetics and Molecular Biology 2 25%
Pharmacology, Toxicology and Pharmaceutical Science 1 13%
Psychology 1 13%
Chemistry 1 13%
Other 0 0%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 November 2017.
All research outputs
#10,749,728
of 12,122,714 outputs
Outputs from Cancer Cell International
#336
of 443 outputs
Outputs of similar age
#237,270
of 282,980 outputs
Outputs of similar age from Cancer Cell International
#15
of 38 outputs
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So far Altmetric has tracked 443 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.