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Reagent and laboratory contamination can critically impact sequence-based microbiome analyses

Overview of attention for article published in BMC Biology, November 2014
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Title
Reagent and laboratory contamination can critically impact sequence-based microbiome analyses
Published in
BMC Biology, November 2014
DOI 10.1186/s12915-014-0087-z
Pubmed ID
Authors

Susannah J Salter, Michael J Cox, Elena M Turek, Szymon T Calus, William O Cookson, Miriam F Moffatt, Paul Turner, Julian Parkhill, Nicholas J Loman, Alan W Walker

Abstract

The study of microbial communities has been revolutionised in recent years by the widespread adoption of culture independent analytical techniques such as 16S rRNA gene sequencing and metagenomics. One potential confounder of these sequence-based approaches is the presence of contamination in DNA extraction kits and other laboratory reagents.

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X Demographics

The data shown below were collected from the profiles of 238 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2,751 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 43 2%
United Kingdom 20 <1%
Canada 8 <1%
Germany 7 <1%
Brazil 6 <1%
Belgium 4 <1%
South Africa 4 <1%
Sweden 4 <1%
France 3 <1%
Other 33 1%
Unknown 2619 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 643 23%
Researcher 556 20%
Student > Master 366 13%
Student > Bachelor 249 9%
Student > Doctoral Student 147 5%
Other 356 13%
Unknown 434 16%
Readers by discipline Count As %
Agricultural and Biological Sciences 962 35%
Biochemistry, Genetics and Molecular Biology 459 17%
Immunology and Microbiology 214 8%
Medicine and Dentistry 173 6%
Environmental Science 138 5%
Other 262 10%
Unknown 543 20%