Title |
Sub-minimum inhibitory concentration of rifampin: a potential risk factor for resuscitation of Mycobacterium tuberculosis
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Published in |
Antimicrobial Resistance & Infection Control, November 2017
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DOI | 10.1186/s13756-017-0273-1 |
Pubmed ID | |
Authors |
Shahin Pourazar Dizaji, Alireza Taala, Morteza Masoumi, Nayereh Ebrahimzadeh, Abolfazl Fateh, Seyed Davar Siadat, Farzam Vaziri |
Abstract |
Mycobacterium tuberculosis possesses five resuscitation-promoting factors, Rpf A to E, which are required for the resuscitation of dormancy in mycobacteria. This study explores the transcriptional profile of all five rpfs of M. tuberculosis, in response to sub-MIC concentration of rifampin, in multidrug and mono-rifampin resistant clinical isolates. Thirteen multidrug and two rifampin mono resistant clinical isolates were analyzed. Drug susceptibility testing and determination of MIC were performed. The relative expression of rpfs was measured, by real-time quantitative PCR. A significant upregulation of relative expression (p < 0.05) was observed, as follows: 7/15(46.66%); 5/15(33.33%); 9/15(60%); 10/15(66.66%) and 9/15(60%) in rpfA, rpfB, rpfC, rpfD and rpfE, respectively. Our results showed that the rpfs could be overexpressed in some extent in the presence of sub-MIC concentration of rifampin in multidrug and mono drug resistant M. tuberculosis. These results highlight the potential risk of sub-MIC rifampin concentrations, as a risk factor for tuberculosis reactivation. |
X Demographics
Geographical breakdown
Country | Count | As % |
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India | 1 | 20% |
United Kingdom | 1 | 20% |
Colombia | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 80% |
Science communicators (journalists, bloggers, editors) | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Postgraduate | 4 | 13% |
Student > Doctoral Student | 3 | 10% |
Student > Bachelor | 3 | 10% |
Other | 3 | 10% |
Professor | 2 | 7% |
Other | 6 | 20% |
Unknown | 9 | 30% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 20% |
Immunology and Microbiology | 3 | 10% |
Agricultural and Biological Sciences | 2 | 7% |
Medicine and Dentistry | 2 | 7% |
Nursing and Health Professions | 1 | 3% |
Other | 5 | 17% |
Unknown | 11 | 37% |