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Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections

Overview of attention for article published in Virology Journal, November 2014
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  • Good Attention Score compared to outputs of the same age (68th percentile)
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Mentioned by

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4 tweeters

Citations

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34 Dimensions

Readers on

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29 Mendeley
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Title
Protease 2A induces stress granule formation during coxsackievirus B3 and enterovirus 71 infections
Published in
Virology Journal, November 2014
DOI 10.1186/s12985-014-0192-1
Pubmed ID
Authors

Shuo Wu, Yan Wang, Lexun Lin, Xiaoning Si, Tianying Wang, Xiaoyan Zhong, Lei Tong, Ying Luan, Yang Chen, Xiaoyu Li, Fengmin Zhang, Wenran Zhao, Zhaohua Zhong

Abstract

BackgroundStress granules (SGs) are granular aggregates in the cytoplasm that are formed under a variety of stress situations including viral infection. Previous studies indicate that poliovirus, a member of Picornaviridae, can induce SG formation. However, the exact mechanism by which the picornaviruses induce SG formation is unknown.MethodThe localization of SG markers in cells infected with coxsackievirus B3 (CVB3) or enterovirus 71 (EV71) and in cells expressing each viral protein was determined via immunofluorescence assays or plasmid transfection. Eight plasmids expressing mutants of the 2A protease (2Apro) of CVB3 were generated using a site-directed mutagenesis strategy. The cleavage efficiencies of eIF4G by CVB3 2Apro, and its mutants were determined via western blotting assays.ResultsIn this study, we found that CVB3 infection induced SG formation, as evidenced by the co-localization of some accepted SG markers in viral infection-induced granules. Furthermore, we identified that 2Apro of CVB3 was the key viral component that triggered SG formation. A 2Apro mutant with the G122E mutation, which exhibited very low cleavage efficiency toward eIF4G, significantly attenuated its capacity for SG induction, indicating that the protease activity was required for 2Apro to initiate SG formation. Finally, we observed that SGs also formed in EV71-infected cells. Expression of EV71 2Apro alone was also sufficient to cause SG formation.ConclusionBoth CVB3 and EV71 infections can induce SG formation, and 2Apro plays a crucial role in the induction of SG formation during these infections. This finding may help us to better understand how picornaviruses initiate the SG response.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
India 1 3%
Unknown 27 93%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 9 31%
Student > Master 7 24%
Researcher 3 10%
Professor > Associate Professor 2 7%
Other 2 7%
Other 3 10%
Unknown 3 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 34%
Agricultural and Biological Sciences 10 34%
Pharmacology, Toxicology and Pharmaceutical Science 1 3%
Medicine and Dentistry 1 3%
Unknown 7 24%

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2014.
All research outputs
#1,930,036
of 5,058,381 outputs
Outputs from Virology Journal
#421
of 1,366 outputs
Outputs of similar age
#52,085
of 168,327 outputs
Outputs of similar age from Virology Journal
#27
of 56 outputs
Altmetric has tracked 5,058,381 research outputs across all sources so far. This one has received more attention than most of these and is in the 61st percentile.
So far Altmetric has tracked 1,366 research outputs from this source. They receive a mean Attention Score of 3.3. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 168,327 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.