↓ Skip to main content

Changes of intestinal microbiota composition and diversity in very low birth weight infants related to strategies of NEC prophylaxis: protocol for an observational multicentre pilot study

Overview of attention for article published in Pilot and Feasibility Studies, November 2017
Altmetric Badge

Mentioned by

twitter
2 tweeters

Citations

dimensions_citation
6 Dimensions

Readers on

mendeley
46 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Changes of intestinal microbiota composition and diversity in very low birth weight infants related to strategies of NEC prophylaxis: protocol for an observational multicentre pilot study
Published in
Pilot and Feasibility Studies, November 2017
DOI 10.1186/s40814-017-0195-y
Pubmed ID
Authors

Stefan Kurath-Koller, Christine Moissl-Eichinger, Gregor Gorkiewicz, Raimund Kraschl, Claudia Kanduth, Barbara Hopfer, Berndt Urlesberger, Bernhard Resch

Abstract

At the Division of Neonatology, Department of Paediatrics, Medical University Graz, a unique regimen of necrotizing enterocolitis (NEC) prophylaxis in preterm infants showing a < 1500 g birth weight (i.e. very low birth weight, VLBW) is used. The regimen includes oral antibiotic and antifungal therapy and probiotic preparations as well as a standardised feeding regimen. The incidence of NEC in preterm infants treated by this regimen has been shown to be lower, reflecting 0.7% when treatment was initiated on the first day of life, compared to international incidence rates (5.1%). However, the impact of the prophylaxis regimen on the intestinal microbiome is yet unknown. We here report the protocol of an observational multicentre STROBE compliant pilot study in VLBW preterm infants. Research will compare three groups as defined by different NEC prophylaxis regimens. Each centre will provide 20 infants. Stool samples will be collected every 2 days throughout the first 2 weeks of life. Samples will be stored at - 80 °C until 16S-rRNA sequencing. 16S-rRNA genes will be amplified using suitable primers (specific for bacteria, fungi and archaea) and prepared for MiSeq Sequencing. Analyses will be performed using public analysis-pipelines, such as Mothur and Qiime, thus allowing an analysis of high-throughput community sequencing data. Abundance and composition changes in intestinal microbiota will be compared between the groups throughout the first 2 weeks of life. Different surroundings at the three participating study centres, including contacts to care takers and parents, as well as feeding or medication all might influence intestinal microbiota composition and abundance. In the planned sequel study, this should be kept in mind and a more standardised process ought to be established. However, the results obtained from the presented pilot study will display the burden of bias and help to establish a more strict protocol for the future. Trial has been registered with the German Registry for Clinical Trials (registry ID DRKS00009290).

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 46 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 10 22%
Student > Bachelor 7 15%
Student > Master 7 15%
Student > Ph. D. Student 4 9%
Student > Postgraduate 3 7%
Other 6 13%
Unknown 9 20%
Readers by discipline Count As %
Medicine and Dentistry 13 28%
Agricultural and Biological Sciences 5 11%
Biochemistry, Genetics and Molecular Biology 4 9%
Immunology and Microbiology 3 7%
Nursing and Health Professions 2 4%
Other 5 11%
Unknown 14 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 November 2017.
All research outputs
#9,732,724
of 12,172,179 outputs
Outputs from Pilot and Feasibility Studies
#248
of 294 outputs
Outputs of similar age
#240,955
of 336,361 outputs
Outputs of similar age from Pilot and Feasibility Studies
#34
of 38 outputs
Altmetric has tracked 12,172,179 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 294 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.1. This one is in the 8th percentile – i.e., 8% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 336,361 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one is in the 5th percentile – i.e., 5% of its contemporaries scored the same or lower than it.