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Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene

Overview of attention for article published in BMC Research Notes, November 2017
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Title
Evaluation of the effect of a floxed Neo cassette within the dystroglycan (Dag1) gene
Published in
BMC Research Notes, November 2017
DOI 10.1186/s13104-017-2926-9
Pubmed ID
Authors

Francesca Sciandra, Bianca Maria Scicchitano, Giulia Signorino, Maria Giulia Bigotti, Barbara Tavazzi, Francesca Lombardi, Manuela Bozzi, Gigliola Sica, Bruno Giardina, Sandra Blaess, Andrea Brancaccio

Abstract

Dystroglycan (DG) is an adhesion complex formed by two subunits, α-DG and β-DG. In skeletal muscle, DG is part of the dystrophin-glycoprotein complex that is crucial for sarcolemma stability and it is involved in a plethora of muscular dystrophy phenotypes. Due to the important role played by DG in skeletal muscle stability as well as in a wide variety of other tissues including brain and the peripheral nervous system, it is essential to investigate its genetic assembly and transcriptional regulation. Herein, we analyze the effect of the insertion of a floxed neomycin (Neo) cassette within the 3' portion of the universally conserved IG1-intron of the DG gene (Dag1). We analyzed the transcription level of Dag1 and the expression of the DG protein in skeletal muscle of targeted mice compared to wild-type and we did not find any alterations that might be attributed to the gene targeting. However, we found an increase of the cross-sectional areas of tibialis anterior that might have some physiological significance that needs to be assessed in the future. Moreover, in targeted mice the skeletal muscle morphology and its regeneration capacity after injury did not show any evident alterations. We confirmed that the targeting of Dag1 with a floxed Neo-cassette did not produce any gross undesired effects.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 5 50%
Student > Ph. D. Student 2 20%
Student > Doctoral Student 1 10%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 50%
Agricultural and Biological Sciences 2 20%
Neuroscience 1 10%
Unknown 2 20%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 November 2017.
All research outputs
#10,797,931
of 12,177,594 outputs
Outputs from BMC Research Notes
#2,216
of 2,696 outputs
Outputs of similar age
#279,871
of 336,525 outputs
Outputs of similar age from BMC Research Notes
#210
of 276 outputs
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We're also able to compare this research output to 276 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.