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Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential

Overview of attention for article published in BMC Biotechnology, December 2014
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (92nd percentile)

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1 news outlet
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2 X users
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1 patent

Citations

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138 Dimensions

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159 Mendeley
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Title
Mesenchymal stem cell spheroids exhibit enhanced in-vitro and in-vivo osteoregenerative potential
Published in
BMC Biotechnology, December 2014
DOI 10.1186/s12896-014-0105-9
Pubmed ID
Authors

Yuichiro Yamaguchi, Jun Ohno, Ayako Sato, Hirofumi Kido, Tadao Fukushima

Abstract

BackgroundMesenchymal stem cells (MSCs) are a favored cell source for regenerative medicine because of their multilinage potential. However, the conventional monolayer technique used to culture MSCs, inadequately overcomes their low differentiation capacity. Culture of MSCs in multicellular spheroids, more accurately mimics the in-vivo microenvironment; thus, resolving this problem. In this study, we assessed whether the osteoregeneartive potential of MSC spheroids is greater than that of monolayer MSCs.ResultsMSC spheroids were generated from rat MSCs (rMSCs) using low-binding plates. Real-time reverse transcription-polymerase chain reaction and immunocytochemical analysis indicated that osteogenic properties were accelerated in MSC spheroids compared with monolayer rMSCs when treated with an osteoblast-inducer reagent for 7 days. Moreover, increased calcium deposition was visualized in MSC spheroids using Alizarin red staining. In a rat calvarial defect model, micro-computed tomography and histological assays showed that MSC spheroid-engrafted defects experienced enhanced bone regeneration.ConclusionsOur in-vitro and in-vivo results reveal that MSCs in the spheroid culture exhibit enhanced osteoregenerative efficiency compared with monolayer MSCs.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 159 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 2 1%
United Kingdom 1 <1%
Colombia 1 <1%
Unknown 155 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 38 24%
Researcher 28 18%
Student > Master 25 16%
Student > Doctoral Student 15 9%
Student > Bachelor 12 8%
Other 14 9%
Unknown 27 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 31 19%
Biochemistry, Genetics and Molecular Biology 28 18%
Medicine and Dentistry 23 14%
Engineering 21 13%
Chemistry 5 3%
Other 19 12%
Unknown 32 20%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 December 2021.
All research outputs
#2,320,012
of 22,653,392 outputs
Outputs from BMC Biotechnology
#67
of 935 outputs
Outputs of similar age
#34,763
of 359,303 outputs
Outputs of similar age from BMC Biotechnology
#2
of 25 outputs
Altmetric has tracked 22,653,392 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 935 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 359,303 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 25 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.