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Diesel exhaust particle exposure in vitro impacts T lymphocyte phenotype and function

Overview of attention for article published in Particle and Fibre Toxicology, December 2014
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  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Above-average Attention Score compared to outputs of the same age and source (52nd percentile)

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4 tweeters

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Title
Diesel exhaust particle exposure in vitro impacts T lymphocyte phenotype and function
Published in
Particle and Fibre Toxicology, December 2014
DOI 10.1186/s12989-014-0074-0
Pubmed ID
Authors

Marina Pierdominici, Angela Maselli, Serena Cecchetti, Antonella Tinari, Arianna Mastrofrancesco, Michela Alfè, Valentina Gargiulo, Carlo Beatrice, Gabriele Di Blasio, Giulia Carpinelli, Elena Ortona, Antonello Giovannetti, Silvana Fiorito

Abstract

BackgroundDiesel exhaust particles (DEP) are major constituents of ambient air pollution and their adverse health effect is an area of intensive investigations. With respect to the immune system, DEP have attracted significant research attention as a factor that could influence allergic diseases interfering with cytokine production and chemokine expression. With this exception, scant data are available on the impact of DEP on lymphocyte homeostasis. Here, the effects of nanoparticles from Euro 4 (E4) and Euro 5 (E5) light duty diesel engines on the phenotype and function of T lymphocytes from healthy donors were evaluated.MethodsT lymphocytes were isolated from peripheral blood obtained from healthy volunteers and subsequently stimulated with different concentration (from 0.15 to 60 ¿g/ml) and at different time points (from 24 h to 9 days) of either E4 or E5 particles. Immunological parameters, including apoptosis, autophagy, proliferation levels, mitochondrial function, expression of activation markers and cytokine production were evaluated by cellular and molecular analyses.ResultsDEP exposure caused a pronounced autophagic-lysosomal blockade, thus interfering with a key mechanism involved in the maintaining of T cell homeostasis. Moreover, DEP decreased mitochondrial membrane potential but, unexpectedly, this effect did not result in changes of the apoptosis and/or necrosis levels, as well as of intracellular content of adenosine triphosphate (ATP). Finally, a down-regulation of the expression of the alpha chain of the interleukin (IL)-2 receptor (i.e., the CD25 molecule) as well as an abnormal Th1 cytokine expression profile (i.e., a decrease of IL-2 and interferon (IFN)-¿ production) were observed after DEP exposure. No differences between the two compounds were detected in all studied parameters.ConclusionsOverall, our data identify functional and phenotypic T lymphocyte parameters as relevant targets for DEP cytotoxicity, whose impairment could be detrimental, at least in the long run, for human health, favouring the development or the progression of diseases such as autoimmunity and cancer.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
Unknown 59 98%

Demographic breakdown

Readers by professional status Count As %
Researcher 14 23%
Student > Master 9 15%
Student > Ph. D. Student 8 13%
Student > Doctoral Student 5 8%
Other 3 5%
Other 9 15%
Unknown 12 20%
Readers by discipline Count As %
Medicine and Dentistry 10 17%
Agricultural and Biological Sciences 9 15%
Immunology and Microbiology 4 7%
Engineering 4 7%
Biochemistry, Genetics and Molecular Biology 3 5%
Other 16 27%
Unknown 14 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 April 2019.
All research outputs
#8,444,378
of 14,596,192 outputs
Outputs from Particle and Fibre Toxicology
#220
of 431 outputs
Outputs of similar age
#131,098
of 299,829 outputs
Outputs of similar age from Particle and Fibre Toxicology
#16
of 36 outputs
Altmetric has tracked 14,596,192 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 431 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.5. This one is in the 48th percentile – i.e., 48% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 299,829 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.