Title |
An autophagosome-based therapeutic vaccine for HBV infection: a preclinical evaluation
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Published in |
Journal of Translational Medicine, December 2014
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DOI | 10.1186/s12967-014-0361-4 |
Pubmed ID | |
Authors |
Meng Xue, Fei Fan, Lei Ding, Jingyu Liu, Shu Su, Pengfei Yin, Meng Cao, Wei Zhao, Hong-ming Hu, Lixin Wang |
Abstract |
BackgroundFor more than 240 million chronic HBV carriers worldwide, effective therapeutic HBV vaccines are urgently needed. Recently, we demonstrated that autophagosomes were efficient antigens carriers and capable to cross-prime robust T-cell responses and mediate regression of multiple established tumors. Here we tested whether autophagosomes derived from HBV expressing cells could also function as a therapeutic vaccine.MethodsWe generated an autophagosome-based HBV vaccine from HBV-expressing hepatoma cells and examined its ability to induce polyvalent anti-HBV T-cell responses and therapeutic efficacy in mouse models that mimics acute and chronic HBV infection in human.ResultsWhen compared to the vaccine based on recombinant HBsAg, autophagosome-based HBV vaccine cross-primed multi-specific anti-HBV T-cell responses and significantly reduced HBV replication and HBcAg expression in livers of both acute and chronic mouse models. Therapeutic effect of this HBV vaccine depended on anti-HBV CD8+ effector T cells and associated with increased HBsAg and HBcAg specific IFN-¿ producing T cells in the chronic mouse model.ConclusionsThese results indicated that autophagosome-based HBV vaccine could effectively suppress the HBV replication, clear the HBV infected hepatocytes, and break the HBV tolerance in mouse model. The potential clinical application of autophagosome-based HBV vaccine is discussed. |
X Demographics
Geographical breakdown
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United States | 2 | 50% |
Unknown | 2 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 100% |
Mendeley readers
Geographical breakdown
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France | 1 | 5% |
Unknown | 18 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 5 | 26% |
Researcher | 5 | 26% |
Student > Doctoral Student | 2 | 11% |
Lecturer > Senior Lecturer | 2 | 11% |
Lecturer | 1 | 5% |
Other | 1 | 5% |
Unknown | 3 | 16% |
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Medicine and Dentistry | 5 | 26% |
Immunology and Microbiology | 4 | 21% |
Agricultural and Biological Sciences | 3 | 16% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
Psychology | 1 | 5% |
Other | 1 | 5% |
Unknown | 3 | 16% |